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Structural and functional basis for lipid synergy on the activity of the antibacterial peptide ABC transporter McjD
Authors:
Shahid
Mehmood
(University of Oxford)
,
Valentina
Corradi
(University of Calgary)
,
Hassan
Choudhury
(Imperial College London)
,
Rohanah
Hussain
(Diamond Light Source)
,
Patrick
Becker
(Imperial College London)
,
Danny
Axford
(Diamond Light Source)
,
Severine
Zirah
(Museum National d'Histoire Naturelle)
,
Sylvie
Rebuffat
(Museum National d'Histoire Naturelle)
,
D. Peter
Tieleman
(University of Calgary)
,
Carol V.
Robinson
(Oxford Univiersity)
,
Konstantinos
Beis
(Diamond Light Source)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Journal Of Biological Chemistry
State:
Published (Approved)
Published:
August 2016
Diamond Proposal Number(s):
10136
,
14484

Abstract: The lipid bilayer is a dynamic environment that consists of a mixture of lipids with different properties that regulate the function of membrane proteins; these lipids are either annular, masking the protein hydrophobic surface, or specific lipids, essential for protein function. In this study, using tandem mass spectrometry, we have identified specific lipids associated with the Escherichia coli ABC transporter McjD, which translocates the antibacterial peptide MccJ25. Using non-denaturing mass spectrometry, we show that McjD in complex with MccJ25 survives the gas-phase. Partial delipidation of McjD resulted in reduced ATPase activity and thermostability as shown by Circular Dichroism, both of which could be restored upon addition of defined E. coli lipids. We have resolved a phosphatidylglycerol lipid associated with McjD at 3.4 Å resolution, while molecular dynamic simulations carried out in different lipid environments assessed the binding of specific lipids to McjD. Combined, our data show a synergistic effect of zwitterionic and negatively charged lipids on the activity of McjD; the zwitterionic lipids provide structural stability to McjD whereas the negatively charged lipids are essential for its function.
Journal Keywords: lipid, membrane transport; membrane protein; structure-function; structural biology; ABC transporter; non-denaturing mass spectrometry; molecular dynamics; delipidation; circular dichroism
Subject Areas:
Biology and Bio-materials
Instruments:
B23-Circular Dichroism
,
I24-Microfocus Macromolecular Crystallography
Added On:
28/08/2016 14:31
Documents:
21656.full.pdf
Discipline Tags:
Structural biology
Life Sciences & Biotech
Technical Tags:
Diffraction
Spectroscopy
Macromolecular Crystallography (MX)
Circular Dichroism (CD)