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A De Novo Virus-Like Topology for Synthetic Virions

DOI: 10.1021/jacs.6b05751 DOI Help

Authors: James Noble (National Physical Laboratory) , Emiliana De Santis (National Physical Laboratory) , Jascindra Ravi (National Physical Laboratory) , Baptiste Lamarre (National Physical Laboratory) , Valeria Castelletto (National Physical Laboratory, UK) , Judith Mantell (University of Bristol) , Santanu Ray (SET, University of Brighton) , Maxim G. Ryadnov (National Physical Laboratory)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of The American Chemical Society

State: Published (Approved)
Published: September 2016
Diamond Proposal Number(s): 12321 , 10083 , 10068

Open Access Open Access

Abstract: A de novo topology of virus-like assembly is reported. The design is a trifaceted coiled-coil peptide helix, which self-assembles into ultrasmall, monodisperse, anionic virus-like shells that encapsulate and transfer both RNA and DNA into human cells. Unlike existing artificial systems, these shells share the same physical characteristics of viruses being anionic, nonaggregating, abundant, hollow, and uniform in size, while effectively mediating gene silencing and transgene expression. These are the smallest virus-like structures reported to date, both synthetic and native, with the ability to adapt and transfer small and large nucleic acids. The design thus offers a promising solution for engineering bespoke artificial viruses with desired functions.

Subject Areas: Biology and Bio-materials, Chemistry

Instruments: B21-High Throughput SAXS , B23-Circular Dichroism