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A self-assembling fluorescent dipeptide conjugate for cell labelling

DOI: 10.1016/j.colsurfb.2015.04.062 DOI Help

Authors: Steven Kirkham (University of Reading) , Ian W. Hamley (School of Chemistry, Pharmacy and Food Biosciences, University of Reading) , Andrew M. Smith (School of Chemistry, Pharmacy and Food Biosciences, University of Reading) , Ricardo M. Gouveia (Institute of Genetic Medicine, Newcastle University) , Che J. Connon (Institute of Genetic Medicine, Newcastle University) , Mehedi Reza (Department of Applied Physics, Aalto University School of Science) , Janne Ruokolainen (Department of Applied Physics, Aalto University School of Science)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Colloids And Surfaces B: Biointerfaces , VOL 137 , PAGES 104 - 108

State: Published (Approved)
Published: January 2016
Diamond Proposal Number(s): 10007

Abstract: Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in peri-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers.

Journal Keywords: Peptides; Peptide conjugates; Self-assembly; Fluorescence

Subject Areas: Chemistry, Biology and Bio-materials


Instruments: B21-High Throughput SAXS

Added On: 01/10/2016 02:52

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