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Spectroscopy reveals that ethyl esters interact with proteins in wine

DOI: 10.1016/j.foodchem.2016.08.133 DOI Help

Authors: Mattia Di Gaspero (University of Padova) , Paolo Ruzza (Institute of Biomolecular Chemistry of CNR, Padua Unit) , Rohanah Hussain (Diamond Light Source) , Simone Vincenzi (University of Padova) , Barbara Biondi (Institute of Biomolecular Chemistry of CNR, Padua Unit) , Diana Gazzola (Department of Agronomy, Food, Natural Resources Animals and Environment (DAFNAE), University of Padua) , Giuliano Siligardi (Diamond Light Source) , Andrea Curioni (Department of Agronomy, Food, Natural Resources Animals and Environment (DAFNAE), University of Padua)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Food Chemistry

State: Published (Approved)
Published: September 2016
Diamond Proposal Number(s): 11540 , 12086

Abstract: Impairment of wine aroma after vinification is frequently associated to bentonite treatments and this can be the result of protein removal, as recently demonstrated for ethyl esters. To evaluate the existence of an interaction between wine proteins and ethyl esters, the effects induced by these fermentative aroma compounds on the secondary structure and stability of VVTL1, a Thaumatin-like protein purified from wine, was analyzed by Synchrotron Radiation Circular Dichroism (SRCD) spectroscopy. The secondary structure of wine VVTL1 was not strongly affected by the presence of selected ethyl esters. In contrast, VVTL1 stability was slightly increased by the addition of ethyl-octanoate, -decanoate and -dodecanoate, but decreased by ethyl-hexanoate. This indicates the existence of an interaction between VVTL1 and at least some aroma compounds produced during fermentation. The data suggest that proteins removal from wine by bentonite can result in indirect removal of at least some aroma compounds associated with them.

Journal Keywords: Aroma; Ethyl esters; Proteins; Spectroscopy; SRCD; VVTL1; Wine

Subject Areas: Food Science, Chemistry, Biology and Bio-materials


Instruments: B23-Circular Dichroism

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