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Applications of the BLEND Software to Crystallographic Data from Membrane Proteins

DOI: 10.1007/978-3-319-35072-1_9 DOI Help

Authors: Isabel Moraes (Diamond Light Source) , Pierre Aller (Diamond Light Source) , Tian Geng (Membrane Protein Laboratory, Diamond Light Source) , Gwyndaf Evans (Diamond Light Source) , James Foadi (Diamond Light Source)
Co-authored by industrial partner: No

Type: Book Chapter

State: Published (Approved)
Published: August 2016

Abstract: X-ray diffraction from crystals of membrane proteins very often yields incomplete datasets due to, among other things, severe radiation damage. Multiple crystals are thus required to form complete datasets, provided the crystals themselves are isomorphous. Selection and combination of data from multiple crystals is a difficult and tedious task that can be facilitated by purpose-built software. BLEND, in the CCP4 suite of programs for macromolecular crystallography (MX), has been created exactly for this reason. In this chapter the program is described and its workings illustrated by means of data from two membrane proteins.

Journal Keywords: Blend; Multiple crystals; Membrane proteins; Data scaling; Data merging; Isomorphism; Cluster analysis; Radiation damage

Subject Areas: Biology and Bio-materials


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