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Exploiting Microbeams for Membrane Protein Structure Determination

DOI: 10.1007/978-3-319-35072-1_8 DOI Help

Authors: Anna J. Warren (Diamond Light Source) , Danny Axford (Diamond Light Source) , Neil G. Paterson (Diamond Light Source) , Robin L. Owen (Diamond Light Source)
Co-authored by industrial partner: No

Type: Book Chapter

State: Published (Approved)
Published: August 2016

Open Access Open Access

Abstract: A reproducible, and sample independent means of predictably obtaining large, well-ordered crystals has proven elusive in macromolecular crystallography. In the structure determination pipeline, crystallisation often proves to be a rate-limiting step, and the process of obtaining even small or badly ordered crystals can prove time-consuming and laborious. This is particularly true in the field of membrane protein crystallography and this is reflected in the limited number of unique membrane protein structures deposited in the protein data bank (less than 650 by June 2016 – http://​blanco.​biomol.​uci.​edu/​mpstruc). Over recent years the requirement for, and time and cost associated with obtaining, large crystals has been partially alleviated through the development of beamline instrumentation allowing data collection, and structure solution, from ever-smaller crystals. Advances in several areas have led to a step change in what might be considered achievable during a synchrotron trip over the last decade. This chapter will briefly review the current status of the field, the tools available to ease data collection and processing, and give some examples of exploitation of these for membrane protein microfocus macromolecular crystallography.

Journal Keywords: Microfocus macromolecular crystallography; Membrane proteins; In situ data collection; Instrumentation; X-ray microtomography

Subject Areas: Biology and Bio-materials, Physics, Technique Development

Instruments: I24-Microfocus Macromolecular Crystallography

Added On: 17/11/2016 10:19


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