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In situ formation of the first proteinogenically functionalized [TeW 6 O 24 O 2 (Glu)] 7− structure reveals unprecedented chemical and geometrical features of the Anderson-type cluster

DOI: 10.1039/C6CC07004C DOI Help

Authors: Christian Molitor (Universität Wien, Fakultät für Chemie, Institut für Biophysikalische Chemie) , Aleksandar Bijelic (Universität Wien, Fakultät für Chemie, Institut für Biophysikalische Chemie) , Annette Rompel (Universität Wien, Fakultät für Chemie, Institut für Biophysikalische Chemie)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Chem. Commun. , VOL 52 , PAGES 12286 - 12289

State: Published (Approved)
Published: September 2016
Diamond Proposal Number(s): 8476

Open Access Open Access

Abstract: The chemistry of polyoxometalates (POMs) in a protein environment is an almost unexplored but highly relevant research field as important biological and pharmacological attributes of certain POMs are based on their interactions with proteins. We report on the A-type Anderson–Evans polyoxotungstate, [TeW6O24]6− (TEW), mediated crystallization of Coreopsis grandiflora aurone synthase (cgAUS1) using ∼0.24 mM protein and 1.0 mM TEW. The 1.78 Å crystal structure reveals the covalent binding of TEW to the protein under the formation of an unprecedented polyoxotungstate cluster, [TeW6O24O2(Glu)]7− (GluTEW). The polyoxotungstate–protein complex exhibits the first covalent bond between a protein and the A-type Anderson–Evans cluster, an archetype where up to now no hybrid structures exist. The polyoxotungstate is modified at two of its six addenda tungsten atoms, which covalently bind to the carboxylic oxygen atoms of glutamic acid (Glu157), leading to W–O distances of ∼2.35 Å. This ligand substitution reaction is accompanied by a reduction of the coordination number of two μ3 polyoxotungstate oxygen atoms. This is so far unique since all known hybridizations of the Anderson–Evans POM with organic units have been obtained via the functionalization of the B-type Anderson–Evans structure through its bridging oxygen atoms. The structure reported here proves the reactivity of this POM archetype's addenda atoms as it has been administered into the protein solution as a pre-assembled cluster. Moreover, the novel cluster [TeW6O24O2(Glu)]7− displays the great versatility of the Anderson–Evans POM class.

Subject Areas: Chemistry

Instruments: I04-1-Macromolecular Crystallography (fixed wavelength)

Added On: 23/11/2016 11:16


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