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Crystal Dehydration in Membrane Protein Crystallography

DOI: 10.1007/978-3-319-35072-1_6 DOI Help

Authors: Juan Sanchez-weatherby (Diamond Light Source) , Isabel Moraes (Diamond Light Source; Imperial College London)
Co-authored by industrial partner: No

Type: Book Chapter

State: Published (Approved)
Published: August 2016

Open Access Open Access

Abstract: Crystal dehydration has been successfully implemented to facilitate the structural solution of a number of soluble and membrane protein structures over the years. This chapter will present the currently available tools to undertake controlled crystal dehydration, focusing on some successful membrane protein cases. Also discussed here will be some practical considerations regarding membrane protein crystals and the relationship between different techniques in order to help researchers to select the most suitable technique for their projects.

Journal Keywords: Crystal dehydration; HC1; Free mounting system; In situ; Membrane proteins; Relative humidity

Subject Areas: Technique Development, Biology and Bio-materials

Diamond Offline Facilities: Membrane Protein Laboratory (MPL)
Technical Areas: