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Crystal structure of Schistosoma mansoni adenosine phosphorylase/5’-methylthioadenosine phosphorylase and its importance on adenosine salvage pathway

DOI: 10.1371/journal.pntd.0005178 DOI Help

Authors: Juliana Roberta Torini (Universidade de São Paulo) , Jose Brandao-Neto (Diamond Light Source) , Ricardo Demarco (Universidade de São Paulo) , Humberto D' Muniz Pereira (Universidade de São Paulo)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Plos Neglected Tropical Diseases , VOL 10

State: Published (Approved)
Published: December 2016
Diamond Proposal Number(s): 11175 , 5969

Open Access Open Access

Abstract: Schistosoma mansoni do not have de novo purine pathways and rely on purine salvage for their purine supply. It has been demonstrated that, unlike humans, the S. mansoni is able to produce adenine directly from adenosine, although the enzyme responsible for this activity was unknown. In the present work we show that S. mansoni 5´-deoxy-5´-methylthioadenosine phosphorylase (MTAP, E.C. is capable of use adenosine as a substrate to the production of adenine. Through kinetics assays, we show that the Schistosoma mansoni MTAP (SmMTAP), unlike the mammalian MTAP, uses adenosine substrate with the same efficiency as MTA phosphorolysis, which suggests that this enzyme is part of the purine pathway salvage in S. mansoni and could be a promising target for anti-schistosoma therapies. Here, we present 13 SmMTAP structures from the wild type (WT), including three single and one double mutant, and generate a solid structural framework for structure description. These crystal structures of SmMTAP reveal that the active site contains three substitutions within and near the active site when compared to it mammalian counterpart, thus opening up the possibility of developing specific inhibitors to the parasite MTAP. The structural and kinetic data for 5 substrates reveal the structural basis for this interaction, providing substract for inteligent design of new compounds for block this enzyme activity.

Journal Keywords: Adenosine; Adenine; Schistosoma mansoni; Phosphates; Phosphorylases; Purines; Nucleosides; Crystal structure

Diamond Keywords: Schistosomiasis

Subject Areas: Biology and Bio-materials, Medicine

Instruments: I02-Macromolecular Crystallography , I04-1-Macromolecular Crystallography (fixed wavelength) , I04-Macromolecular Crystallography

Added On: 14/12/2016 11:49


Discipline Tags:

Infectious Diseases Disease in the Developing World Health & Wellbeing Structural biology Drug Discovery Life Sciences & Biotech Parasitology

Technical Tags:

Diffraction Macromolecular Crystallography (MX)