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Polyelectrolyte pH-responsive protein-containing nanoparticles: the physico-chemical supramolecular approach

DOI: 10.1021/acs.langmuir.6b03778 DOI Help

Authors: Anna Riabtseva (Institute of Macromolecular Chemistry) , Leonid I. Kaberov (Institute of Macromolecular Chemistry) , Jan Kucka (Institute of Macromolecular Chemistry) , Anna Yurevna Bogomolova (Institute of Macromolecular Chemistry) , Petr Stepanek (Institute of Macromolecular Chemistry) , Sergey Filippov (Institute of Macromolecular Chemistry) , Martin Hruby (Institute of Macromolecular Chemistry)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Langmuir

State: Published (Approved)
Published: January 2017

Abstract: We show physico-chemical colloidal and self-assembly background behind novel efficient pH-sensitive nanocontainers based on the FDA-approved anionic polymer Eudragit L100-55 (poly(methacrylic acid-co-ethyl acrylate) 1:1) and non-ionic surfactant Brij98. The features of interaction between Eudragit L100-55 and Brij98 at different pH and their optimal ratio for nanoparticles formation were studied using isothermal titration calorimetry (ITC). Influence of the polymer-to-surfactant ratio on the size and structure of particles were studied at different pH values using dynamic light scattering (DLS) and small-angle X-ray scattering (SAXS) methods. It was shown that stable nanoparticles are formed at acidic pH at polymer-to-surfactant molar ratios from 1:43 to 1:139. Trypsin was successfully encapsulated into Eudragit/Brij98 nanoparticles as a model bioactive component. Loading efficiency was determined by labeling of trypsin with radioactive iodine-125. Eudragit/Brij98 nanoparticles effectively protected trypsin against pepsin digestion. Results showed that trypsin encapsulated into novel pH-sensitive nanocontainers retained more than 50% of its activity after treatment with pepsin compared with non-encapsulated trypsin. The described concept will contribute to both understanding principles and design of next generation nanocontainers.

Journal Keywords: pH-sensitive polymers; Eudragit; nanocontainers; SAXS; ITC; DLS; trypsin

Subject Areas: Chemistry, Medicine, Materials

Instruments: B21-High Throughput SAXS

Added On: 09/01/2017 09:05

Discipline Tags:

Drug Delivery Physical Chemistry Health & Wellbeing Chemistry Materials Science Nanoscience/Nanotechnology Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)