Synchrotron infrared near-field spectroscopy in photothermal mode

Authors: Chris S. Kelley (Diamond Light Source) , Mark D. Frogley (Diamond Light Source) , Ann Fitzpatrick (Diamond Light Source) , Katia Wehbe (Diamond Light Source) , Paul Donaldson (Central Laser Facility, Research Complex at Harwell) , Gianfelice Cinque (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Spectroscopy Europe , VOL 28 , PAGES 18 - 21

State: Published (Approved)
Published: November 2016

Open Access Open Access

Abstract: Near-field infrared (IR) spectroscopy is revolutionising molecular imaging by allowing vibrational spectroscopic analysis at the sub-micrometre scale. We recently developed the world’s first near-field IR photothermal microscope using synchrotron radiation (SR), which uniquely spans a spectral range from the near-IR to the far-IR/THz. Here we demonstrate the capability of the near-field method to probe polymer microspheres within a protein matrix, and we present the first IRSR photothermal near-field Fourier transform infrared (FT-IR) spectrum from within an individual biological cell, which establishes the feasibility of hyperspectral mapping at sub-micrometre resolution in a practical timescale. Photothermal near-field spectroscopy provides both depth sensitivity and IR molecular specificity, which is ideal for organic matter/biological samples. In addition, SR gives seamless and ultra-broadband spectral coverage superior to the bandwidth of commercially available lasers.

Subject Areas: Technique Development, Biology and Bio-materials

Instruments: B22-Multimode InfraRed imaging And Microspectroscopy

Added On: 09/01/2017 13:26


Discipline Tags:

Technique Development - Life Sciences & Biotech Life Sciences & Biotech

Technical Tags:

Spectroscopy Infrared Spectroscopy Synchtron-based Fourier Transform Infrared Spectroscopy (SR-FTIR)