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The structure of a calcium-dependent phosphoinositide-specific phospholipase C from Pseudomonas sp. 62186, the first from a Gram-negative bacterium

DOI: 10.1107/S2059798316019616 DOI Help

Authors: Olga V. Moroz (University of York) , Elena Blagova (University of York) , Andrey A. Lebedev (STFC Rutherford Appleton Laboratory) , Allan Nørgaard (Novozymes A/S) , Dorotea R. Segura (Novozymes A/S) , Thomas H. Blicher (Novozymes A/S) , Jesper Brask (Novozymes A/S) , Keith S. Wilson (University of York)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section D Structural Biology , VOL 73 , PAGES 32 - 44

State: Published (Approved)
Published: January 2017
Diamond Proposal Number(s): 9948

Abstract: Bacterial phosphoinositide-specific phospholipases C (PI-PLCs) are the smallest members of the PI-PLC family, which includes much larger mammalian enzymes responsible for signal transduction as well as enzymes from protozoan parasites, yeast and plants. Eukaryotic PI-PLCs have calcium in the active site, but this is absent in the known structures of Gram-positive bacteria, where its role is instead played by arginine. In addition to their use in a number of industrial applications, the bacterial enzymes attract special interest because they can serve as convenient models of the catalytic domains of eukaryotic enzymes for in vitro activity studies. Here, the structure of a PI-PLC from Pseudomonas sp. 62186 is reported, the first from a Gram-negative bacterium and the first of a native bacterial PI-PLC with calcium present in the active site. Solution of the structure posed particular problems owing to the low sequence identity of available homologous structures. Its dependence on calcium for catalysis makes this enzyme a better model for studies of the mammalian PI-PLCs than the previously used calcium-independent bacterial PI-PLCs.

Journal Keywords: phospholipase C; X-ray structure; calcium-dependent; phosphoinositol-specific; Gram-negative bacterium; Pseudomonas sp. 62186

Subject Areas: Biology and Bio-materials, Chemistry

Instruments: I03-Macromolecular Crystallography