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Single-cell analysis using Fourier transform infrared microspectroscopy

DOI: 10.1080/05704928.2016.1250214 DOI Help

Authors: James Doherty (University of Manchester; Diamond Light Source) , Gianfelice Cinque (Diamond Light Source) , Peter Gardner (University of Manchester)
Co-authored by industrial partner: No

Type: Magazine Article
Magazine: Applied Spectroscopy Reviews

State: Published (Approved)
Published: October 2016

Abstract: Fourier transform infrared spectroscopy (FTIR) is a well-established, non-destructive method of obtaining chemical information from biological samples such as tissues and cells. This review focuses specifically on the development of infrared spectroscopic microanalysis at the single-cell level. Technological developments, including that of the infrared microscope, synchrotron radiation FTIR, and focal plane array detectors, and their impact on the field are discussed along with the various data processing procedures that are currently used to extract meaningful information. There is then an emphasis on live cell infrared (IR) imaging, including developments in water correction and microfluidic device design. The review concludes with look to future directions, highlighting the potential impact of quantum cascade lasers.

Journal Keywords: FTIR; infrared; live cell imaging; microspectroscopy; quantum cascade lasers (QCLs); single cells

Subject Areas: Technique Development, Biology and Bio-materials


Instruments: B22-Multimode InfraRed imaging And Microspectroscopy

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