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Procedures for cryogenic X-ray ptychographic imaging of biological samples

DOI: 10.1107/S2052252516020029 DOI Help

Authors: M. Yusuf (University College London; Research Complex at Harwell; Brunel University London) , F. Zhang (University College London (UCL); Research Complex at Harwell; Southern University of Science and Technology) , B. Chen (University College London; Research Complex at Harwell) , A. Bhartiya (University College London; Research Complex at Harwell) , K. Cunnea (Research Complex at Harwell) , U. Wagner (Diamond Light Source) , F. Cacho-nerin (Diamond Light Source) , J. Schwenke (University College London; Research Complex at Harwell) , I. K. Robinson (University College London; Research Complex at Harwell; Brookhaven National Laboratory)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Iucrj , VOL 4

State: Published (Approved)
Published: March 2017

Open Access Open Access

Abstract: Biological sample-preparation procedures have been developed for imaging human chromosomes under cryogenic conditions. A new experimental setup, developed for imaging frozen samples using beamline I13 at Diamond Light Source, is described. This manuscript describes the equipment and experimental procedures as well as the authors' first ptychographic reconstructions using X-rays.

Journal Keywords: human chromosomes; cell nuclei; chromosome imaging; X-ray ptychography; plunge freezing

Subject Areas: Technique Development, Biology and Bio-materials


Instruments: I13-1-Coherence

Documents:
lz5012.pdf