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X-ray phase microtomography with a single grating for high-throughput investigations of biological tissue

DOI: 10.1364/BOE.8.001257 DOI Help

Authors: Marie-christine Zdora (Diamond Light Source) , Joan Vila-comamala (Diamond Light Source) , Georg Schulz (University of Basel) , Anna Khimchenko (University of Basel) , Alexander Hipp (Helmholtz-Zentrum Geesthacht) , Andrew C. Cook (University College London Institute of Cardiovascular Science) , Daniel Dilg (University College London Institute of Cardiovascular Science) , Christian David (Paul Scherrer Institut) , Christian Gr├╝nzweig (Paul Scherrer Institut) , Christoph Rau (Diamond Light Source; University of Manchester; Northwestern University) , Pierre Thibault (University of Southampton) , Irene Zanette (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Biomedical Optics Express , VOL 8

State: Published (Approved)
Published: January 2017
Diamond Proposal Number(s): 13164

Open Access Open Access

Abstract: The high-throughput 3D visualisation of biological specimens is essential for studying diseases and developmental disorders. It requires imaging methods that deliver high-contrast, high-resolution volumetric information at short sample preparation and acquisition times. Here we show that X-ray phase-contrast tomography using a single grating can provide a powerful alternative to commonly employed techniques, such as high-resolution episcopic microscopy (HREM). We present the phase tomography of a mouse embryo in paraffin obtained with an X-ray single-grating interferometer at I13-2 Beamline at Diamond Light Source and discuss the results in comparison with HREM measurements. The excellent contrast and quantitative density information achieved non-destructively and without staining using a simple, robust setup make X-ray single-grating interferometry an optimum candidate for high-throughput imaging of biological specimens as an alternative for existing methods like HREM.

Subject Areas: Biology and Bio-materials


Instruments: I13-2-Diamond Manchester Imaging