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Structural characterization of EasH (Aspergillus japonicus) – an oxidase involved in cycloclavine biosynthesis

DOI: 10.1039/C6CC08438A DOI Help

Authors: Dorota Jakubczyk (John Innes Centre) , Lorenzo Caputi (John Innes Centre) , Clare E. M. Stevenson (John Innes Centre) , David M. Lawson (John Innes Centre) , Sarah E. O'Connor (John Innes Centre)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Chemical Communications , VOL 52 , PAGES 14306 - 14309

State: Published (Approved)
Published: November 2016
Diamond Proposal Number(s): 9475

Open Access Open Access

Abstract: Aj_EasH is a non-heme iron- and α-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. The three dimensional structure of Aj_EasH (2.2 Å resolution) reported here provides insight into the mechanism of this unusual and complex reaction.

Journal Keywords: EasH; ergot alkaloids; non-heme iron oxidase; alpha-keto-glutarate; crystal structure

Diamond Keywords: Enzymes

Subject Areas: Biology and Bio-materials, Chemistry, Medicine


Instruments: I02-Macromolecular Crystallography

Added On: 09/03/2017 09:38

Documents:
c6cc08438a.pdf

Discipline Tags:

Health & Wellbeing Biochemistry Catalysis Chemistry Structural biology Drug Discovery Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)