Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes

DOI: 10.1007/s11120-017-0386-7

Authors: June Southall (Institute of Molecular cell and Systems Biology, University of Glasgow) , Sarah L. Henry (Biomedical Engineering, School of Engineering, University of Glasgow) , Alastair T. Gardiner (Institute of Molecular cell and Systems Biology, University of Glasgow) , Aleksander W. Roszak (Institute of Molecular cell and Systems Biology, University of Glasgow) , William Mullen (Institute of Cardiovascular and medical Sciences, University of Glasgow) , Anne-Marie Carey (Center for Innovations in Medicine, The Biodesign Institute, Arizona State University, Arizona, USA) , Sharon M. Kelly (Institute of Molecular cell and Systems Biology, University of Glasgow) , Claire Ortmann De Percin Northumberland (Institute of Molecular cell and Systems Biology, University of Glasgow) , Richard J. Cogdell (Institute of Molecular cell and Systems Biology, University of Glasgow)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Photosynthesis Research

State: Published (Approved)
Published: May 2017
Diamond Proposal Number(s): 8659 , 11651

Abstract: Rhodopseudomonas palustris is a species of purple photosynthetic bacteria that has a multigene family of puc genes that encode the alpha and beta apoproteins, which form the LH2 complexes. In order to try to understand which spectroscopic form of LH2 these different genes produce we have adopted a genetic dissection strategy. This paper presents a characterisation of one of the deletion mutants generated in this program, the pucBAd only mutant. This mutant produces an unusual spectroscopic form of LH2 that only has a single large NIR absorption band at 800nm. Spectroscopic and pigment analyses on this complex suggest that it has basically a similar overall structure as that of the wild-type HL LH2 complex. The mutant has the unique phenotype where the mutant LH2 complex is only produced when cells are grown at LL. At HL the mutant only produces the LH1-RC core complex.

Journal Keywords: Photosynthesis; purple bacteria; puc genes; light-harvesting; LH2

Diamond Keywords: Bacteria; Photosynthesis

Subject Areas: Biology and Bio-materials, Energy


Instruments: I02-Macromolecular Crystallography , I03-Macromolecular Crystallography , I04-Macromolecular Crystallography

Added On: 27/03/2017 16:26

Documents:
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Discipline Tags:

Genetics Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)