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Complex pectin metabolism by gut bacteria reveals novel catalytic functions
Authors:
Didier
Ndeh
(Newcastle University)
,
Artur
Rogowski
(Newcastle University)
,
Alan
Cartmell
(Newcastle University)
,
Ana S.
Luis
(Newcastle University)
,
Arnaud
Basle
(Newcastle University)
,
Joseph
Gray
(Newcastle University)
,
Immacolata
Venditto
(CIISA, Faculdade de Medicina Veterinaria, Universidade de Lisboa)
,
Jonathon
Briggs
(Newcastle University)
,
Xiaoyang
Zhang
(Newcastle University)
,
Aurore
Labourel
(Newcastle University)
,
Nicolas
Terrapon
(Aix-Marseille University)
,
Fanny
Buffetto
(INRA)
,
Sergey
Nepogodiev
(John Innes Centre Norwich Research Park)
,
Yao
Xiao
(University of Michigan Medical School)
,
Robert A.
Field
(John Innes Centre Norwich Research Park)
,
Yanping
Zhu
(The University of Georgia)
,
Malcolm A.
O’neill
(The University of Georgia)
,
Breeanna R.
Urbanowicz
(The University of Georgia)
,
William S.
York
(The University of Georgia)
,
Gideon J.
Davies
(University of York)
,
D. Wade
Abbott
(Lethbridge Research Centre)
,
Marie-Christine
Ralet
(INRA)
,
Eric C.
Martens
(University of Michigan Medical School)
,
Bernard
Henrissat
(INRA; King Abdulaziz University)
,
Harry J.
Gilbert
(Newcastle University)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Nature
, VOL 411
State:
Published (Approved)
Published:
March 2017
Diamond Proposal Number(s):
1960
,
7854
,
9948
Abstract: The metabolism of carbohydrate polymers drives microbial diversity in the human gut microbiota. It is unclear, however, whether bacterial consortia or single organisms are required to depolymerize highly complex glycans. Here we show that the gut bacterium Bacteroides thetaiotaomicron uses the most structurally complex glycan known: the plant pectic polysaccharide rhamnogalacturonan-II, cleaving all but 1 of its 21 distinct glycosidic linkages. The deconstruction of rhamnogalacturonan-II side chains and backbone are coordinated to overcome steric constraints, and the degradation involves previously undiscovered enzyme families and catalytic activities. The degradation system informs revision of the current structural model of rhamnogalacturonan-II and highlights how individual gut bacteria orchestrate manifold enzymes to metabolize the most challenging glycan in the human diet.
Journal Keywords: Glycobiology; Microbiology
Diamond Keywords: Gut Microbiota; Bacteria; Enzymes
Subject Areas:
Biology and Bio-materials,
Chemistry
Instruments:
I02-Macromolecular Crystallography
,
I04-1-Macromolecular Crystallography (fixed wavelength)
,
I24-Microfocus Macromolecular Crystallography
Added On:
28/03/2017 12:03
Discipline Tags:
Health & Wellbeing
Biochemistry
Chemistry
Structural biology
Life Sciences & Biotech
Technical Tags:
Diffraction
Macromolecular Crystallography (MX)