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Low-dose fixed-target serial synchrotron crystallography

DOI: 10.1107/S2059798317002996 DOI Help

Authors: Robin L. Owen (Diamond Light Source) , Danny Axford (Diamond Light Source) , Darren A. Sherrell (Diamond Light Source) , Anling Kuo (University of Toronto) , Oliver P. Ernst (University of Toronto) , Eike Schulz (Max Planck Institute for the Structure and Dynamics of Matter) , R. J. Dwayne Miller (Max Planck Institute for the Structure and Dynamics of Matter; University of Toronto) , Henrike Mueller-werkmeister (University of Toronto; Max Planck Institute for the Structure and Dynamics of Matter)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section D Structural Biology , VOL 73 , PAGES 373 - 378

State: Published (Approved)
Published: April 2017
Diamond Proposal Number(s): 11175

Open Access Open Access

Abstract: The development of serial crystallography has been driven by the sample requirements imposed by X-ray free-electron lasers. Serial techniques are now being exploited at synchrotrons. Using a fixed-target approach to high-throughput serial sampling, it is demonstrated that high-quality data can be collected from myoglobin crystals, allowing room-temperature, low-dose structure determination. The combination of fixed-target arrays and a fast, accurate translation system allows high-throughput serial data collection at high hit rates and with low sample consumption.

Journal Keywords: macromolecular crystallography; serial synchrotron crystallography; fixed target; room-temperature crystallography; crystallography on a chip; low dose

Subject Areas: Technique Development, Biology and Bio-materials


Instruments: I24-Microfocus Macromolecular Crystallography

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