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Electron Bio-Imaging Centre (eBIC): the UK national research facility for biological electron microscopy

DOI: 10.1107/S2059798317007756 DOI Help

Authors: Daniel K. Clare (Diamond Light Source) , C. Alistair Siebert (Diamond Light Source) , Corey Hecksel (Diamond Light Source) , Christoph Hagen (University of Oxford) , Valerie Mordhorst (University of Oxford) , Michael Grange (University of Oxford) , Alun W. Ashton (Diamond Light Source) , Martin A. Walsh (Diamond Light Source) , Kay Grunewald (Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford) , Helen R. Saibil (Diamond Light Source; Birkbeck College) , Dave I. Stuart (Diamond Light Source; University of Oxford) , Peijun Zhang (Diamond Light Source; University of Oxford)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section D Structural Biology , VOL 73 , PAGES 488 - 495

State: Published (Approved)
Published: June 2017

Open Access Open Access

Abstract: The recent resolution revolution in cryo-EM has led to a massive increase in demand for both time on high-end cryo-electron microscopes and access to cryo-electron microscopy expertise. In anticipation of this demand, eBIC was set up at Diamond Light Source in collaboration with Birkbeck College London and the University of Oxford, and funded by the Wellcome Trust, the UK Medical Research Council (MRC) and the Biotechnology and Biological Sciences Research Council (BBSRC) to provide access to high-end equipment through peer review. eBIC is currently in its start-up phase and began by offering time on a single FEI Titan Krios microscope equipped with the latest generation of direct electron detectors from two manufacturers. Here, the current status and modes of access for potential users of eBIC are outlined. In the first year of operation, 222 d of microscope time were delivered to external research groups, with 95 visits in total, of which 53 were from unique groups. The data collected have generated multiple high- to intermediate-resolution structures (2.8–8 Å), ten of which have been published. A second Krios microscope is now in operation, with two more due to come online in 2017. In the next phase of growth of eBIC, in addition to more microscope time, new data-collection strategies and sample-preparation techniques will be made available to external user groups. Finally, all raw data are archived, and a metadata catalogue and automated pipelines for data analysis are being developed.

Journal Keywords: Electron Bio-Imaging Centre; eBIC; cryo-EM; cryo-ET; user facilities

Subject Areas: Technique Development

Diamond Offline Facilities: Electron Bio-Imaging Centre (eBIC)
Instruments: Krios I-Titan Krios I at Diamond