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Serial Synchrotron X-Ray Crystallography (SSX)

DOI: 10.1007/978-1-4939-7000-1_10 DOI Help

Authors: Kay Diederichs (Universit├Ąt Konstanz) , Meitian Wang (Swiss Light Source)
Co-authored by industrial partner: No

Type: Book Chapter

State: Published (Approved)
Published: June 2017

Abstract: Prompted by methodological advances in measurements with X-ray free electron lasers, it was realized in the last two years that traditional (or conventional) methods for data collection from crystals of macromolecular specimens can be complemented by synchrotron measurements on microcrystals that would individually not suffice for a complete data set. Measuring, processing, and merging many partial data sets of this kind requires new techniques which have since been implemented at several third-generation synchrotron facilities, and are described here. Among these, we particularly focus on the possibility of in situ measurements combined with in meso crystal preparations and data analysis with the XDS package and auxiliary programs.

Journal Keywords: Serial synchrotron crystallography (SSX); Microcrystal; Lipidic cubic phase (LCP); In meso in situ; Room temperature (RT); Cryogenic temperature; Data collection; Data quality; Merging; XDS; XSCALE

Subject Areas: Physics, Technique Development


Instruments: I24-Microfocus Macromolecular Crystallography

Other Facilities: Swiss Light Source