Publication

Article Metrics

Citations


Online attention

Hard X-ray submicrometer tomography of human brain tissue at Diamond Light Source

DOI: 10.1088/1742-6596/849/1/012030 DOI Help

Authors: A. Khimchenko (University of Basel) , C. Bikis (University of Basel) , G. Schulz (University of Basel) , M.-c. Zdora (Diamond Light Source) , I. Zanette (Diamond Light Source) , Joan Vila-comamala (Diamond Light Source) , G. Schweighauser (Basel University Hospital) , J. Hench (Basel University Hospital) , S. E. Hieber (University of Basel) , H. Deyhle (University of Basel) , P. Thalmann (University of Basel) , B. Muller (University of Basel)
Co-authored by industrial partner: No

Type: Conference Paper
Conference: X-Ray Microscopy Conference 2016 (XRM 2016)
Peer Reviewed: No

State: Published (Approved)
Published: June 2017

Open Access Open Access

Abstract: There is a lack of the necessary methodology for three-dimensional (3D) investigation of soft tissues with cellular resolution without staining or tissue transformation. Synchrotron radiation based hard X-ray in-line phase contrast tomography using single-distance phase reconstruction (SDPR) provides high spatial resolution and density contrast for the visualization of individual cells using a standard specimen preparation and data reconstruction. In this study, we demonstrate the 3D characterization of a formalin-fixed paraffin-embedded (FFPE) human cerebellum specimen by SDPR at the Diamond-Manchester Imaging Branchline I13-2 (Diamond Light Source, UK) at pixel sizes down to 0.45 μm. The approach enables visualization of cerebellar layers (Stratum moleculare and Stratum granulosum), the 3D characterization of individual cells (Purkinje, stellate and granule cells) and can even resolve some subcellular structures (nucleus and nucleolus of Purkinje cells). The tomographic results are qualitatively compared to hematoxylin and eosin (H&E) stained histological sections. We demonstrate the potential benefits of hard X-ray microtomography for the investigations of biological tissues in comparison to conventional histology.

Subject Areas: Biology and Bio-materials, Technique Development


Instruments: I13-2-Diamond Manchester Imaging

Documents:
pdf.pdf