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High-speed fixed-target serial virus crystallography

DOI: 10.1038/nmeth.4335 DOI Help

Authors: Philip Roedig (Deutsches Elektronen-Synchrotron DESY) , Helen M. Ginn (University of Oxford) , Tim Pakendorf (Deutsches Elektronen-Synchrotron DESY) , Geoff Sutton (University of Oxford) , Karl Harlos (Wellcome Trust Centre for Human Genetics, University of Oxford) , Thomas S. Walter (University of Oxford) , Jan Meyer (Deutsches Elektronen-Synchrotron DESY) , Pontus Fischer (Deutsches Elektronen-Synchrotron DESY) , Ramona Duman (Diamond Light Source) , Ismo Vartiainen (University of Eastern Finland) , Bernd Reime (Deutsches Elektronen-Synchrotron DESY) , Martin Warmer (Deutsches Elektronen-Synchrotron DESY) , Aaron S. Brewster (Lawrence Berkeley National Laboratory) , Iris D. Young (Lawrence Berkeley National Laboratory) , Tara Michels-Clark (Lawrence Berkeley National Laboratory) , Nicholas K. Sauter (Lawrence Berkeley National Laboratory) , Abhay Kotecha (University of Oxford) , James Kelly (University of Leeds; Pirbright Institute) , David J. Rowlands (University of Leeds) , Marcin Sikorsky (SLAC National Accelerator Laboratory) , Silke Nelson (SLAC National Accelerator Laboratory) , Daniel S. Damiani (SLAC National Accelerator Laboratory) , Roberto Alonso-Mori (SLAC National Accelerator Laboratory) , Jingshan Ren (University of Oxford) , Elizabeth E. Fry (Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford) , Christian David (Paul Scherrer Institut) , David I. Stuart (Diamond Light Source; Wellcome Trust Centre for Human Genetics, University of Oxford) , Armin Wagner (Diamond Light Source) , Alke Meents (Deutsches Elektronen-Synchrotron DESY)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Methods , VOL 8

State: Published (Approved)
Published: June 2017

Abstract: We report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with the high-speed Roadrunner goniometer for sample delivery, we were able to determine the crystal structures of the picornavirus bovine enterovirus 2 (BEV2) and the cytoplasmic polyhedrosis virus type 18 polyhedrin, with total data collection times of less than 14 and 10 min, respectively. Our method requires only micrograms of sample and should therefore broaden the applicability of serial femtosecond crystallography to challenging projects for which only limited sample amounts are available. By synchronizing the sample exchange to the XFEL repetition rate, our method allows for most efficient use of the limited beam time available at XFELs and should enable a substantial increase in sample throughput at these facilities.

Journal Keywords: Nanocrystallography; Proteins; Virology

Diamond Keywords: Viruses

Subject Areas: Biology and Bio-materials, Technique Development

Facility: SLAC National Accelerator Laboratory

Added On: 23/06/2017 08:51

Discipline Tags:

Pathogens Infectious Diseases Health & Wellbeing Technique Development - Life Sciences & Biotech Structural biology Life Sciences & Biotech

Technical Tags: