Best practices for managing large CryoEM facilities

DOI: 10.1016/j.jsb.2017.07.011

Authors: Bart Alewijnse (NeCEN, Leiden University) , Alun W. Ashton (Diamond Light Source) , Melissa G. Chambers (Harvard Medical School) , Songye Chen (California Institute of Technology) , Anchi Cheng (New York Structural Biology Center) , Mark Ebrahim (The Rockefeller University) , Edward T. Eng (New York Structural Biology Center) , Wim J. H. Hagen (EMBL Heidelberg) , Abraham J. Koster (NeCEN, Leiden University) , Claudia S. López (Oregon Health & Science University) , Natalya Lukoyanova (ISMB/Birkbeck) , Joaquin Ortega (McGill University) , Ludovic Renault (NeCEN, Leiden University) , Steve Reyntjens (FEI Company/Thermo Fisher Scientific) , William J. Rice (New York Structural Biology Center) , Giovanna Scapin (Merck & Co, Inc) , Raymond Schrijver (FEI Company/Thermo Fisher Scientific) , Alistair Siebert (Diamond Light Source) , Scott M. Stagg (Florida State University) , Valerie Grum-tokars (Northwestern University) , Elizabeth R. Wright (Emory University) , Shenping Wu (Yale University) , Zhiheng Yu (HHMI Janelia Research Campus) , Z. Hong Zhou (University of California) , Bridget Carragher (New York Structural Biology Center) , Clinton S. Potter (New York Structural Biology Center)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Structural Biology

State: Published (Approved)
Published: August 2017

Abstract: This paper provides an overview of the discussion and presentations from the Workshop on the Management of Large CryoEM Facilities held at the New York Structural Biology Center, New York, NY on February 6–7, 2017. A major objective of the workshop was to discuss best practices for managing cryoEM facilities. The discussions were largely focused on supporting single-particle methods for cryoEM and topics included: user access, assessing projects, workflow, sample handling, microscopy, data management and processing, and user training.

Journal Keywords: Cryotem; Automation; Facility management; User training; Single-particle; Data management

Subject Areas: Technique Development


Technical Areas: