Publication

Microfluidic devices fabricated using soft lithography for the study of protein structures using synchrotron radiation circular dichroism

Authors: Jerome Charmet (University of Warwick) , Christian Bortolini (University of Cambridge) , Davor Copic (University of Cambridge) , Itzel Condado Morales (University of Cambridge) , Yuewen Zhang (University of Cambridge) , Tamas Javorfi (Diamond Light Source) , Rohanah Hussain (Diamond Light Source) , Giuliano Siligardi (Diamond Light Source) , Tuomas Knowles (University of Cambridge)
Co-authored by industrial partner: No

Type: Conference Paper
Conference: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Peer Reviewed: Yes

State: Published (Approved)
Published: October 2016
Diamond Proposal Number(s): 14750 , 15005 , 15592 , 16203

Abstract: The combination of microfluidic with Synchrotron Radiation Circular Dichroism (SRCD) is highly promising for the study of protein interactions and misfolding in real time. However, due to the strong absorption of many materials in the far-UV region, the fabrication of microfluidic devices has been limited to fused silica devices. Here we present two device architectures, fabricated using conventional PDMS-based soft-lithography, compatible with SRCD

Subject Areas: Technique Development, Biology and Bio-materials, Engineering


Instruments: B23-Circular Dichroism