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Supramolecular Peptide Nanofiber Morphology Affects Mechanotransduction of Stem Cells

DOI: 10.1021/acs.biomac.7b00773 DOI Help

Authors: Elif Arslan (Bilkent University) , Meryem Hatip Koc (Bilkent University) , Ozge Uysal (Bilkent University) , Begum Dikecoglu (Bilkent University) , Ahmet Emin Topal (Bilkent University) , Ruslan Garifullin (Bilkent University; Kazan Federal University) , Alper D. Ozkan (Bilkent University) , Aykutlu Dana (Bilkent University) , Daniel Hermida-merino (ESRF – The European Synchrotron) , Valeria Castelletto (University of Reading) , Charlotte Edwards-gayle (University of Reading) , Sefer Baday (Institute, Istanbul Technical University) , Ian W. Hamley (University of Reading) , Ayse Begum Tekinay (Bilkent University) , Mustafa O. Guler (University of Chicago)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Biomacromolecules

State: Published (Approved)
Published: August 2017
Diamond Proposal Number(s): 14684

Abstract: Chirality and morphology are essential factors for protein function and interactions with other biomacromolecules. Extracellular matrix (ECM) proteins are also similar to other proteins in this sense; however, the complexity of the natural ECM makes it difficult to study these factors at the cellular level. The synthetic peptide nanomaterials harbor great promise in mimicking specific ECM molecules as model systems. In this work, we demonstrate that mechanosensory responses of stem cells are directly regulated by the chirality and morphology of ECM-mimetic peptide nanofibers with strictly controlled characteristics. Structural signals presented on L-amino acid containing cylindrical nanofibers (L-VV) favored the formation of integrin 1-based focal adhesion complexes, which increased the osteogenic potential of stem cells through the activation of nuclear YAP. On the other hand, twisted ribbon-like nanofibers (L-FF and D-FF) guided the cells into round shapes and decreased the formation of focal adhesion complexes resulting in the confinement of YAP proteins in the cytosol and a corresponding decrease in osteogenic potential. Interestingly, the D-form of twisted-ribbon like nanofibers (D-FF) increased the chondrogenic potential of stem cells more than their L-form (L-FF). Our results provide new insights into the importance and relevance of morphology and chirality of nanomaterials in their interactions with cells, and reveal that precise control over the chemical and physical properties of nanostructures can affect stem cell fate even without the incorporation of specific epitopes.

Journal Keywords: Supramolecular peptide nanofibers; mechanotransduction; stem cells; chirality; morphology

Subject Areas: Biology and Bio-materials

Instruments: B21-High Throughput SAXS

Other Facilities: ESRF