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Characterization of Three Druggable Hot-Spots in the Aurora-A/TPX2 Interaction Using Biochemical, Biophysical, and Fragment-Based Approaches

DOI: 10.1021/acschembio.7b00537 DOI Help

Authors: Patrick J. Mcintyre (University of Leicester) , Patrick Collins (Diamond Light Source) , Lukáš Vrzal (University of Chemistry and Technology; Institute of Organic Chemistry and Biochemistry) , Kristian Birchall (LifeArc (Formerly MRC Technology)) , Laurence H. Arnold (LifeArc (Formerly MRC Technology)) , Chido Mpamhanga (LifeArc (Formerly MRC Technology)) , Peter J. Coombs (LifeArc (Formerly MRC Technology)) , Selena G. Burgess (University of Leeds) , Mark W. Richards (University of Leeds) , Anja Winter (University of Leicester) , Václav Veverka (Institute of Organic Chemistry and Biochemistry) , Frank Von Delft (Diamond Light Source) , Andy Merritt (LifeArc (Formerly MRC Technology)) , Richard Bayliss (University of Leeds)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acs Chemical Biology

State: Published (Approved)
Published: October 2017
Diamond Proposal Number(s): 14331

Abstract: The mitotic kinase Aurora-A and its partner protein TPX2 (Targeting Protein for Xenopus kinesin-like protein 2) are overexpressed in cancers, and it has been proposed that they work together as an oncogenic holoenzyme. TPX2 is responsible for activating Aurora-A during mitosis, ensuring proper cell division. Disruption of the interface with TPX2 is therefore a potential target for novel anticancer drugs that exploit the increased sensitivity of cancer cells to mitotic stress. Here, we investigate the interface using coprecipitation assays and isothermal titration calorimetry to quantify the energetic contribution of individual residues of TPX2. Residues Tyr8, Tyr10, Phe16, and Trp34 of TPX2 are shown to be crucial for robust complex formation, suggesting that the interaction could be abrogated through blocking any of the three pockets on Aurora-A that complement these residues. Phosphorylation of Aurora-A on Thr288 is also necessary for high-affinity binding, and here we identify arginine residues that communicate the phosphorylation of Thr288 to the TPX2 binding site. With these findings in mind, we conducted a high-throughput X-ray crystallography-based screen of 1255 fragments against Aurora-A and identified 59 hits. Over three-quarters of these hits bound to the pockets described above, both validating our identification of hotspots and demonstrating the druggability of this protein–protein interaction. Our study exemplifies the potential of high-throughput crystallography facilities such as XChem to aid drug discovery. These results will accelerate the development of chemical inhibitors of the Aurora-A/TPX2 interaction.

Subject Areas: Biology and Bio-materials, Medicine


Instruments: I04-1-Macromolecular Crystallography (fixed wavelength)