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Building the atomic model of a boreal lake virus of unknown fold in a 3.9 Å cryo-EM map

DOI: 10.1016/j.jsb.2017.10.010 DOI Help

Authors: Luigi De Colibus (University of Oxford) , David I. Stuart (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Structural Biology

State: Published (Approved)
Published: October 2017

Open Access Open Access

Abstract: We report here the protocol adopted to build the atomic model of the newly discovered virus FLiP (Flavobacterium infecting, lipid-containing phage) into 3.9 Å cryo-electron microscopy (cryo-EM) maps. In particular, this report discusses the combination of density modification procedures, automatic model building and bioinformatics tools applied to guide the tracing of the major capsid protein (MCP) of this newly discovered virus. The protocol outlined here may serve as a reference for future structural determination by cryo-EM of viruses lacking detectable structural homologues.

Subject Areas: Biology and Bio-materials


Technical Areas:

Documents:
1-s2.0-S1047847717301788-main.pdf