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DNA Ligase C and Prim-PolC participate in base excision repair in mycobacteria

DOI: 10.1038/s41467-017-01365-y DOI Help

Authors: Przemysław Płociński (Institute of Medical Biology, Polish Academy of Sciences) , Nigel C. Brissett (University of Sussex) , Julie Bianchi (University of Sussex) , Anna Brzostek (Institute of Medical Biology, Polish Academy of Sciences) , Małgorzata Korycka-Machała (Institute of Medical Biology, Polish Academy of Sciences) , Andrzej Dziembowski (Institute of Biochemistry and Biophysics, Polish Academy of Sciences) , Jarosław Dziadek (Institute of Medical Biology, Polish Academy of Sciences) , Aidan J. Doherty (University of Sussex)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Communications , VOL 8

State: Published (Approved)
Published: November 2017
Diamond Proposal Number(s): 10088

Open Access Open Access

Abstract: Prokaryotic Ligase D is a conserved DNA repair apparatus processing DNA double-strand breaks in stationary phase. An orthologous Ligase C (LigC) complex also co-exists in many bacterial species but its function is unknown. Here we show that the LigC complex interacts with core BER enzymes in vivo and demonstrate that together these factors constitute an excision repair apparatus capable of repairing damaged bases and abasic sites. The polymerase component, which contains a conserved C-terminal structural loop, preferentially binds to and fills-in short gapped DNA intermediates with RNA and LigC ligates the resulting nicks to complete repair. Components of the LigC complex, like LigD, are expressed upon entry into stationary phase and cells lacking either of these pathways exhibit increased sensitivity to oxidising genotoxins. Together, these findings establish that the LigC complex is directly involved in an excision repair pathway(s) that repairs DNA damage with ribonucleotides during stationary phase.

Journal Keywords: Base excision repair; DNA

Diamond Keywords: Bacteria; Tuberculosis (TB)

Subject Areas: Biology and Bio-materials

Instruments: I02-Macromolecular Crystallography

Added On: 01/11/2017 08:58


Discipline Tags:

Pathogens Infectious Diseases Health & Wellbeing Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)