An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography

DOI: 10.1039/C7LC00812K

Authors: C. G. Burton (Aston University) , Danny Axford (Diamond Light Source) , Andrew Michael John Edwards (Nottingham Trent University) , Richard J. Gildea (Diamond Light Source) , Robert H. Morris (Nottingham Trent University) , Michael I. Newton (Nottingham Trent University) , Allen M. Orville (Diamond Light Source) , Mark Prince (Aston University) , Paul D. Topham (Aston University) , Peter D. Docker (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Lab On A Chip

State: Published (Approved)
Published: November 2017

Abstract: This paper describes the design, development and successful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for randomised in-fluid sample orientation and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facillitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.

Subject Areas: Physics, Technique Development


Instruments: I24-Microfocus Macromolecular Crystallography

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