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An Acoustic On-Chip Goniometer for Room Temperature Macromolecular Crystallography
Authors:
C. G.
Burton
(Aston University)
,
Danny
Axford
(Diamond Light Source)
,
Andrew Michael John
Edwards
(Nottingham Trent University)
,
Richard J.
Gildea
(Diamond Light Source)
,
Robert H.
Morris
(Nottingham Trent University)
,
Michael I.
Newton
(Nottingham Trent University)
,
Allen M.
Orville
(Diamond Light Source)
,
Mark
Prince
(Aston University)
,
Paul D.
Topham
(Aston University)
,
Peter D.
Docker
(Diamond Light Source)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Lab On A Chip
State:
Published (Approved)
Published:
November 2017
Abstract: This paper describes the design, development and successful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for randomised in-fluid sample orientation and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facillitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.
Subject Areas:
Physics,
Technique Development
Instruments:
I24-Microfocus Macromolecular Crystallography
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