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Target identification and mode of action of four chemically divergent drugs against Ebola virus infection

DOI: 10.1021/acs.jmedchem.7b01249 DOI Help

Authors: Jingshan Ren (University of Oxford) , Yuguang Zhao (University of Oxford) , Elizabeth E. Fry (University of Oxford) , David I. Stuart (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Medicinal Chemistry

State: Published (Approved)
Published: December 2017
Diamond Proposal Number(s): 10627

Abstract: Here we show that four chemically divergent approved drugs reported to inhibit Ebolavirus infection, benztropine, bepridil, paroxetine and sertraline, directly interact with the Ebolavirus glycoprotein. Binding of these drugs destabilise the protein, suggesting that this may be the mechanism of inhibition, as reported for the anticancer drug toremifene and the painkiller ibuprofen, which bind in the same large cavity on the glycoprotein. Crystal structures show that the position of binding and the mode of interaction within the pocket vary significantly between these compounds. The binding constants (Kd) determined by thermal shift assay correlate with the protein-inhibitor interactions as well as with the antiviral activities determined by virus cell entry assays, supporting the hypothesis that these drugs inhibit viral entry by binding the glycoprotein and destabilising the pre-fusion conformation. Details of the protein–inhibitor interactions of these complexes and their relation with binding affinity may facilitate the design of more potent inhibitors.

Journal Keywords: Ebolavirus; Marburgvirus; Ebolavirus glycoprotein; Ebolavirus entry inhibitor; benztropine; bepridil; paroxetine; sertraline

Diamond Keywords: Ebola; Viruses

Subject Areas: Biology and Bio-materials, Chemistry, Medicine

Instruments: I02-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography

Added On: 04/01/2018 11:19

Discipline Tags:

Organic Chemistry Life Sciences & Biotech Health & Wellbeing Disease in the Developing World Drug Discovery Infectious Diseases Pathogens Structural biology Chemistry Biochemistry

Technical Tags:

Diffraction Macromolecular Crystallography (MX)