Publication

The long-wavelength macromolecular crystallography beamline I23 at Diamond Light Source

Authors: Armin Wagner (Diamond Light Source) , Ramona Duman (Diamond Light Source) , Kamel El Omari (University of Oxford) , Vinay Grama (Diamond Light Source) , Vitaliy Mykhaylyk (Diamond Light Source)
Co-authored by industrial partner: No

Type: Conference Paper
Conference: Twenty-Fourth Congress and General Assembly of the International Union of Crystallography
Peer Reviewed: No

State: Published (Approved)
Published: August 2017

Abstract: Solving the crystallographic phase problem by single wavelength anomalous diffraction (SAD) directly in the absence of a known protein model similar to the one under investigation and without additional labelling of the protein or nucleic acid has the potential to become the method of choice for phasing macromolecular crystals. This technique, also referred to as native phasing, utilizes the increase of the anomalous signal from sulfur or phosphorus towards their K absorption edges which are around 5 Å and 6 Å, respectively. Over the past years technical developments such as single-photon counting detectors and improved stability from synchrotron sources and beamline equipment have led to an increased number of protein and nucleic acid structures being solved by experimental phasing techniques at longer wavelengths around λ = 2 Å [1].

Journal Keywords: SAD; long wavelength; synchrotron beamline

Subject Areas: Technique Development


Technical Areas: Data acquisition