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Biosynthesis and characterization of copper nanoparticles using Shewanella oneidensis: application for click chemistry

DOI: 10.1002/smll.201703145 DOI Help

Authors: Richard Kimber (University of Manchester) , Edward A. Lewis (University of Manchester) , Fabio Parmeggiani (University of Manchester) , Kurt Smith (University of Manchester) , Heath Bagshaw (University of Manchester) , Toby Starborg (University of Manchester) , Nimisha Joshi (University of Manchester) , Adriana Figueroa (Diamond Light Source) , Gerrit Van Der Laan (Diamond Light Source) , Giannantonio Cibin (Diamond Light Source) , Diego Gianolio (Diamond Light Source) , Sarah J. Haigh (University of Manchester) , Richard A. D. Pattrick (University of Manchester) , Nicholas J. Turner (University of Manchester) , Jonathan R. Lloyd (University of Manchester)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Small , VOL 116 , PAGES 1703145

State: Published (Approved)
Published: January 2018
Diamond Proposal Number(s): 15476 , 16136

Open Access Open Access

Abstract: Copper nanoparticles (Cu-NPs) have a wide range of applications as heterogeneous catalysts. In this study, a novel green biosynthesis route for producing Cu-NPs using the metal-reducing bacterium, Shewanella oneidensis is demonstrated. Thin section transmission electron microscopy shows that the Cu-NPs are predominantly intracellular and present in a typical size range of 20–40 nm. Serial block-face scanning electron microscopy demonstrates the Cu-NPs are well-dispersed across the 3D structure of the cells. X-ray absorption near-edge spectroscopy and extended X-ray absorption fine-structure spectroscopy analysis show the nanoparticles are Cu(0), however, atomic resolution images and electron energy loss spectroscopy suggest partial oxidation of the surface layer to Cu2O upon exposure to air. The catalytic activity of the Cu-NPs is demonstrated in an archetypal “click chemistry” reaction, generating good yields during azide-alkyne cycloadditions, most likely catalyzed by the Cu(I) surface layer of the nanoparticles. Furthermore, cytochrome deletion mutants suggest a novel metal reduction system is involved in enzymatic Cu(II) reduction and Cu-NP synthesis, which is not dependent on the Mtr pathway commonly used to reduce other high oxidation state metals in this bacterium. This work demonstrates a novel, simple, green biosynthesis method for producing efficient copper nanoparticle catalysts.

Journal Keywords: biosynthesis; XAS; bugs

Subject Areas: Environment, Chemistry, Physics

Diamond Offline Facilities: Magnetic Spectroscopy Lab
Instruments: B18-Core EXAFS , I10-Beamline for Advanced Dichroism

Other Facilities: No

Documents:
Kimber_et_al-2018-Small.pdf