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Directed Evolution of Alcohol Dehydrogenase for Improved Stereoselective Redox Transformations of 1-Phenylethane-1,2-diol and Its Corresponding Acyloin

DOI: 10.1021/acs.biochem.8b00055 DOI Help

Authors: Emil Hamnevik (Uppsala University) , Dirk Maurer (Uppsala University) , Thilak Reddy Enugala (Uppsala University) , Thao Chu (Uppsala University) , Robin Löfgren (Uppsala University) , Doreen Dobritzsch (Uppsala University) , Mikael Widersten (Uppsala University)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Biochemistry

State: Published (Approved)
Published: February 2018
Diamond Proposal Number(s): 11171

Abstract: Laboratory evolution of alcohol dehydrogenase produced enzyme variants with improved turnover numbers with a vicinal 1,2-diol and its corresponding hydroxyketone. Crystal structure and transient kinetics analysis aids in rationalizing the new functions of these variants.

Subject Areas: Biology and Bio-materials, Chemistry

Instruments: I04-Macromolecular Crystallography