Article Metrics


Online attention

Pirating conserved phage mechanisms promotes promiscuous staphylococcal pathogenicity island transfer

DOI: 10.7554/eLife.26487 DOI Help

Authors: Janine Bowring (University of Glasgow) , Maan M. Neamah (University of Glasgow; University of Kufa) , Jorge Donderis (3Instituto de Biomedicina de Valencia (IBV-CSIC); CIBER de Enfermedades Raras) , Ignacio Mir-sanchis (Universidad CEU Cardenal Herrera) , Christian Alite (3Instituto de Biomedicina de Valencia (IBV-CSIC); CIBER de Enfermedades Raras) , J. Rafael Ciges-tomas (3Instituto de Biomedicina de Valencia (IBV-CSIC); CIBER de Enfermedades Raras) , Elisa Maiques (3Instituto de Biomedicina de Valencia (IBV-CSIC); CIBER de Enfermedades Raras; Universidad CEU Cardenal Herrera) , Iltyar Medmedov (3Instituto de Biomedicina de Valencia (IBV-CSIC); CIBER de Enfermedades Raras) , Alberto Marina , José R Penadés (University of Glasgow)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Elife , VOL 6

State: Published (Approved)
Published: August 2017
Diamond Proposal Number(s): 14739

Open Access Open Access

Abstract: Targeting conserved and essential processes is a successful strategy to combat enemies. Remarkably, the clinically important Staphylococcus aureus pathogenicity islands (SaPIs) use this tactic to spread in nature. SaPIs reside passively in the host chromosome, under the control of the SaPI-encoded master repressor, Stl. It has been assumed that SaPI de-repression is effected by specific phage proteins that bind to Stl, initiating the SaPI cycle. Different SaPIs encode different Stl repressors, so each targets a specific phage protein for its de-repression. Broadening this narrow vision, we report here that SaPIs ensure their promiscuous transfer by targeting conserved phage mechanisms. This is accomplished because the SaPI Stl repressors have acquired different domains to interact with unrelated proteins, encoded by different phages, but in all cases performing the same conserved function. This elegant strategy allows intra- and inter-generic SaPI transfer, highlighting these elements as one of nature’s most fascinating subcellular parasites.

Subject Areas: Biology and Bio-materials, Chemistry, Medicine

Instruments: I02-Macromolecular Crystallography , I03-Macromolecular Crystallography , I04-1-Macromolecular Crystallography (fixed wavelength) , I04-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography

Other Facilities: ALBA