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Structure of Ljungan virus provides insight into genome packaging of this picornavirus

DOI: 10.1038/ncomms9316 DOI Help

Authors: Ling Zhu (University of Oxford; The Pirbright Institute) , Xiangxi Wang (Chinese Academy of Science) , Jingshan Ren (University of Oxford) , Claudine Porta (University of Oxford; The Pirbright Institute) , Hannah Wenham (The Pirbright Institute) , Jens-ola Ekström (Linnaeus University) , Anusha Panjwani (The Pirbright Institute) , Nick J. Knowles (The Pirbright Institute) , Abhay Kotecha (University of Oxford) , C. Alistair Siebert (University of Oxford) , A. Michael Lindberg (Linnaeus University) , Elizabeth E. Fry (University of Oxford) , Zihe Rao (Tsinghua University; Chinese Academy of Science) , Tobias J. Tuthill (The Pirbright Institute) , David I. Stuart (University of Oxford; Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Communications , VOL 6

State: Published (Approved)
Published: December 2015

Open Access Open Access

Abstract: Picornaviruses are responsible for a range of human and animal diseases, but how their RNA genome is packaged remains poorly understood. A particularly poorly studied group within this family are those that lack the internal coat protein, VP4. Here we report the atomic structure of one such virus, Ljungan virus, the type member of the genus Parechovirus B, which has been linked to diabetes and myocarditis in humans. The 3.78-Å resolution cryo-electron microscopy structure shows remarkable features, including an extended VP1 C terminus, forming a major protuberance on the outer surface of the virus, and a basic motif at the N terminus of VP3, binding to which orders some 12% of the viral genome. This apparently charge-driven RNA attachment suggests that this branch of the picornaviruses uses a different mechanism of genome encapsidation, perhaps explored early in the evolution of picornaviruses.

Journal Keywords: Biophysics; Viral genetics; Virus structures

Subject Areas: Biology and Bio-materials

Diamond Offline Facilities: Electron Bio-Imaging Centre (eBIC)
Instruments: M01-Polara at OPIC (Oxford)

Documents:
ncomms9316.pdf