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Cryo-soft X-ray tomography: using soft X-rays to explore the ultrastructure of whole cells

DOI: 10.1042/ETLS20170086 DOI Help

Authors: Maria Harkiolaki (Diamond Light Source) , Michele C. Darrow (Diamond Light Source) , Matthew C. Spink (Diamond Light Source) , Ewelina Kosior (Diamond Light Source) , Kyle Dent (Diamond Light Source) , Elisabeth Duke (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Emerging Topics In Life Sciences

State: Published (Approved)
Published: March 2018

Open Access Open Access

Abstract: Cryo-soft X-ray tomography is an imaging technique that addresses the need for mesoscale imaging of cellular ultrastructure of relatively thick samples without the need for staining or chemical modification. It allows the imaging of cellular ultrastructure to a resolution of 25–40 nm and can be used in correlation with other imaging modalities, such as electron tomography and fluorescence microscopy, to further enhance the information content derived from biological samples. An overview of the technique, discussion of sample suitability and information about sample preparation, data collection and data analysis is presented here. Recent developments and future outlook are also discussed.

Subject Areas: Biology and Bio-materials, Physics


Instruments: B24-Cryo Soft X-ray Tomography

Documents:
ETLS20170086.full.pdf

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