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Rational design of multi-functional gold nanoparticles with controlled biomolecule adsorption: a multi-method approach for in-depth characterization

DOI: 10.1039/C8NR00973B DOI Help

Authors: Isaac Ojea-Jimenez (European Commission, DG-Joint Research Centre) , Robin Capomaccio (European Commission, DG-Joint Research Centre) , Inês Osório (European Commission, DG-Joint Research Centre) , Dora Mehn (European Commission, DG-Joint Research Centre) , Giacomo Ceccone (European Commission, DG-Joint Research Centre) , Rohanah Hussain (Diamond Light Source) , Giuliano Siligardi (Diamond Light Source) , Pascal Colpo (European Commission, DG-Joint Research Centre) , François Rossi (European Commission, DG-Joint Research Centre) , Douglas Gilliland (European Commission, DG-Joint Research Centre) , Luigi Calzolai (European Commission, DG-Joint Research Centre)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nanoscale , VOL 124

State: Published (Approved)
Published: May 2018
Diamond Proposal Number(s): 11373

Open Access Open Access

Abstract: Multi-functionalized nanoparticles are of great interest in biotechnology and biomedicine, especially for diagnostic and therapeutic purposes. However, at the moment the characterization of complex, multi-functional nanoparticles is still challenging and this hampers the development of advanced nanomaterials for biological applications. In this work, we have designed a model system consisting of gold nanoparticles functionalized with two differentially-terminated poly(ethylene oxide) ligands, providing both “stealth” properties and protein-binding capabilities to the nanoparticles. We use a combination of techniques (Centrifugal Liquid Sedimentation, Dynamic Light Scattering, Flow Field Flow Fractionation, Transmission Electron Microscopy, and Circular Dichroism) to: (i) monitor and quantify the ratios of ligand molecules per nanoparticle; (ii) determine the effect of coating density on non-specific protein adsorption; (iii) to assess the number and structure of the covalently-bound proteins. This article aims at comparing the complementary outcomes from typical and orthogonal techniques used in nanoparticle characterization by employing a versatile nanoparticle-ligands-biomolecule model system.

Subject Areas: Materials, Chemistry, Medicine

Instruments: B23-Circular Dichroism

Added On: 23/05/2018 09:50


Discipline Tags:

Physical Chemistry Chemistry Materials Science Nanoscience/Nanotechnology Life Sciences & Biotech

Technical Tags:

Spectroscopy Circular Dichroism (CD)