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Construction of fluorescent analogs to follow the uptake and distribution of cobalamin (vitamin B12) in bacteria, worms, and plants

DOI: 10.1016/j.chembiol.2018.04.012 DOI Help

Authors: Andrew D. Lawrence (University of Kent) , Emi Nemoto-smith (University of Kent; National Institutes of Health) , Evelyne Deery (University of Kent) , Joseph A. Baker (University of Kent) , Susanne Schroeder (University of Kent) , David G. Brown (University of Kent) , Jennifer M. A. Tullet (University of Kent) , Mark J. Howard (University of Kent) , Ian R. Brown (University of Kent) , Alison G. Smith (University of Cambridge) , Helena I. Boshoff (National Institutes of Health, Bethesda) , Clifton E. Barry (National Institutes of Health) , Martin J. Warren (University of Kent)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Cell Chemical Biology

State: Published (Approved)
Published: May 2018

Abstract: Vitamin B12 is made by only certain prokaryotes yet is required by a number of eukaryotes such as mammals, fish, birds, worms, and Protista, including algae. There is still much to learn about how this nutrient is trafficked across the domains of life. Herein, we describe ways to make a number of different corrin analogs with fluorescent groups attached to the main tetrapyrrole-derived ring. A further range of analogs were also constructed by attaching similar fluorescent groups to the ribose ring of cobalamin, thereby generating a range of complete and incomplete corrinoids to follow uptake in bacteria, worms, and plants. By using these fluorescent derivatives we were able to demonstrate that Mycobacterium tuberculosis is able to acquire both cobyric acid and cobalamin analogs, that Caenorhabditis elegans takes up only the complete corrinoid, and that seedlings of higher plants such as Lepidium sativum are also able to transport B12.

Journal Keywords: vitamin B12; cobalamin; tetrapyrrole; biosynthesis; analogs; fluorescence; trafficking; Mycobacterium tuberculosis; Caenorhabditis elegans; higher plants

Subject Areas: Biology and Bio-materials


Instruments: I04-1-Macromolecular Crystallography (fixed wavelength)