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Pectin conformation in solution

DOI: 10.1021/acs.jpcb.8b04790 DOI Help

Authors: Katerina Alba (University of Huddersfield) , Richard J. Bingham (University of Huddersfield) , Patrick A. Gunning (Quadram Institute Bioscience) , Peter J. Wilde (Quadram Institute Bioscience) , V. Kontogiorgos (University of Huddersfield)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: The Journal Of Physical Chemistry B

State: Published (Approved)
Published: July 2018
Diamond Proposal Number(s): 17074

Abstract: The interplay of degree of methylesterification (DM), pH, temperature, and concentration on the macromolecular interactions of pectin in solution has been explored. Small angle X-ray scattering complemented by atomic force microscopy and molecular dynamics were employed to probe chain dimensions and solution structure. Two length scales have been observed with the first characterising chain clustering with a size ranging between 100-200 nm. The second level of structure arises from single biopolymer chains with radius of gyration between ~6-42 nm. The development of a range of macromolecular dimensions in vitro and in silico shows chain flexibility increases with DM and at acidic pH whereas hydrogen bonding is the responsible thermodynamic driving force for cluster formation. High methyl pectins create structures of lower fractal dimension with less efficient packing. This work unveils pectin conformations covering most of its industrially and biologically relevant environments, enabling rational design of advanced biomaterials based on pectin.

Subject Areas: Biology and Bio-materials, Chemistry


Instruments: B21-High Throughput SAXS