Merging in-solution x-ray and neutron scattering data allows fine structural analysis of membrane–protein detergent complexes

DOI: 10.1021/acs.jpclett.8b01598

Authors: Gaetan Dias Mirandela (University of Strathclyde) , Giulia Tamburrino (University of Dundee) , Miloš T. Ivanović (Saarland University) , Felix M. Strnad (University of Goettingen) , Olwyn Byron (University of Glasgow) , Tim Rasmussen (University of Aberdeen) , Paul A. Hoskisson (University of Strathclyde) , Jochen S. Hub (University of Strathclyde; Saarland University) , Ulrich Zachariae (University of Strathclyde; University of Dundee) , Frank Gabel (University of Strathclyde; Institut Laue-Langevin; University of Grenoble Alpes) , Arnaud Javelle (University of Strathclyde)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: The Journal Of Physical Chemistry Letters

State: Published (Approved)
Published: July 2018
Diamond Proposal Number(s): 15616

Abstract: In-solution small-angle X-ray and neutron scattering (SAXS/SANS) have become popular methods to characterize the structure of membrane proteins, solubilized by either detergents or nanodiscs. SANS studies of protein-detergent complexes usually require deuterium-labeled proteins or detergents, which in turn often lead to problems in their expression or purification. Here, we report an approach whose novelty is the combined analysis of SAXS and SANS data from an unlabeled membrane protein complex in solution in two complementary ways. First, an explicit atomic analysis, including both protein and detergent molecules, using the program WAXSiS, which has been adapted to predict SANS data. Second, the use of MONSA which allows one to discriminate between detergent head- and tail-groups in an ab initio approach. Our approach is readily applicable to any detergent-solubilized protein and provides more detailed structural information on protein–detergent complexes from unlabeled samples than SAXS or SANS alone.

Journal Keywords: Scattering; Peptides and proteins; Molecular modeling; X-ray scattering,Molecules

Subject Areas: Chemistry, Biology and Bio-materials, Technique Development


Instruments: B21-High Throughput SAXS

Other Facilities: Institut Laue-Langevin

Added On: 09/07/2018 11:13

Documents:
acs.56456pclett.pdf

Discipline Tags:

Biochemistry Soft condensed matter physics Technique Development - Life Sciences & Biotech Chemistry Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)