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A computed microtomography method for understanding epiphyseal growth plate fusion

DOI: 10.3389/fmats.2017.00048 DOI Help

Authors: Katherine A. Staines (Edinburgh Napier University) , Kamel Madi (The University of Manchester) , Behzad Javaheri (Royal Veterinary College) , Peter D. Lee (University of Manchester) , Andrew A. Pitsillides (Royal Veterinary College)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Frontiers In Materials , VOL 4

State: Published (Approved)
Published: January 2018
Diamond Proposal Number(s): 13237 , 11076 , 5003

Open Access Open Access

Abstract: The epiphyseal growth plate is a developmental region responsible for linear bone growth, in which chondrocytes undertake a tightly regulated series of biological processes. Concomitant with the cessation of growth and sexual maturation, the human growth plate undergoes progressive narrowing, and ultimately disappears. Despite the crucial role of this growth plate fusion “bridging” event, the precise mechanisms by which it is governed are complex and yet to be established. Progress is hindered by the current methods for growth plate visualization; these are invasive and largely rely on histological procedures. Here, we describe our non-invasive method utilizing synchrotron X-ray computed microtomography for the examination of growth plate bridging, which ultimately leads to its closure coincident with termination of further longitudinal bone growth. We then apply this method to a dataset obtained from a benchtop micro computed tomography scanner to highlight its potential for wide usage. Furthermore, we conduct finite element modeling at the micron-scale to reveal the effects of growth plate bridging on local tissue mechanics. Employment of these 3D analyses of growth plate bone bridging is likely to advance our understanding of the physiological mechanisms that control growth plate fusion.

Journal Keywords: growth plate; mouse; bone; synchrotron; computed tomography; fusion

Diamond Keywords: Bone

Subject Areas: Biology and Bio-materials
Collaborations: Diamond Manchester

Instruments: I13-2-Diamond Manchester Imaging

Added On: 12/07/2018 10:30


Discipline Tags:

Life Sciences & Biotech Biophysics

Technical Tags:

Imaging Tomography