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In situ monitoring and analysis of enamel demineralisation using synchrotron X-ray scattering

DOI: 10.1016/j.actbio.2018.07.027 DOI Help

Authors: Tan Sui (University of Oxford; University of Surrey) , Enrico Salvati (University of Oxford) , Robert A. Harper (University of Birmingham) , Hongjia Zhang (University of Oxford) , Richard M. Shelton (University of Birmingham) , Gabriel Landini (University of Birmingham) , Alexander M. Korsunsky (University of Oxford)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Biomaterialia

State: Published (Approved)
Published: July 2018
Diamond Proposal Number(s): 15981

Abstract: Dental caries is one of the most common chronic diseases that affect human teeth. It often initiates in enamel, undermining its mechanical function and structural integrity. Little is known about the enamel demineralisation process caused by dental caries in terms of the microstructural changes and crystallography of the inorganic mineral phase. To improve the understanding of the carious lesion formation process and to help identify efficient treatments, the evolution of the microstructure at the nano-scale in an artificially induced enamel erosion region was probed using advanced synchrotron small-angle and wide-angle X-ray scattering (SAXS and WAXS). This is the first in vitro and time-resolved investigation of enamel demineralisation using synchrotron X-ray techniques which allows in situ quantification of the microstructure evolution over time in a simulated carious lesion. The analysis revealed that alongside the reduction of mineral volume, a heterogeneous evolution of hydroxyapatite (HAp) crystallites (in terms of size, preferred orientation and degree of alignment) could be observed. It was also found that the rate and direction of dissolution depends on the crystallographic orientation. Based on these findings, a novel conceptual view of the process is put forward that describes the key structural parameters in establishing high fidelity ultrastructure-based numerical models for the simulation of the enamel demineralisation process.

Journal Keywords: Dental caries; Dental demineralisation; Enamel; SAXS; WAXS; in situ analysis

Subject Areas: Biology and Bio-materials

Instruments: B16-Test Beamline

Added On: 19/07/2018 08:44

Discipline Tags:

Dentistry Health & Wellbeing Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS) Wide Angle X-ray Scattering (WAXS)