Structural basis for selective inhibition of immunoglobulin E-receptor interactions by an anti-IgE antibody

DOI: 10.1038/s41598-018-29664-4

Authors: Jiun-Bo Chen (Genomics Research Center, Academia Sinica; King's College London) , Faruk Ramadani (King’s College London; Medical Research Council & Asthma UK Centre in Allergic Mechanisms of Asthma) , Marie O. Y. Pang (King’s College London; Medical Research Council & Asthma UK Centre in Allergic Mechanisms of Asthma) , Rebecca L. Beavil (King’s College London; Medical Research Council & Asthma UK Centre in Allergic Mechanisms of Asthma) , Mary D. Holdom (King’s College London; Medical Research Council & Asthma UK Centre in Allergic Mechanisms of Asthma) , Alkistis N. Mitropoulou (King’s College London; Medical Research Council & Asthma UK Centre in Allergic Mechanisms of Asthma) , Andrew J. Beavil (King’s College London; Medical Research Council & Asthma UK Centre in Allergic Mechanisms of Asthma) , Hannah J. Gould (King’s College London; Medical Research Council & Asthma UK Centre in Allergic Mechanisms of Asthma) , Tse Wen Chang (Genomics Research Center, Academia Sinica) , Brian Sutton (King’s College London; Medical Research Council & Asthma UK Centre in Allergic Mechanisms of Asthma) , James M. Mcdonnell (King’s College London; Medical Research Council & Asthma UK Centre in Allergic Mechanisms of Asthma) , Anna M. Davies (King’s College London; Medical Research Council & Asthma UK Centre in Allergic Mechanisms of Asthma)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Scientific Reports , VOL 8

State: Published (Approved)
Published: August 2018
Diamond Proposal Number(s): 7656

Abstract: Immunoglobulin E (IgE) antibodies play a central role in the allergic response: interaction with FcεRI on mast cells and basophils leads to immediate hypersensitivity reactions upon allergen challenge, while interaction with CD23/FcεRII, expressed on a variety of cells, regulates IgE synthesis among other activities. The receptor-binding IgE-Fc region has recently been found to display remarkable flexibility, from acutely bent to extended conformations, with allosteric communication between the distant FcεRI and CD23 binding sites. We report the structure of an anti-IgE antibody Fab (8D6) bound to IgE-Fc through a mixed protein-carbohydrate epitope, revealing further flexibility and a novel extended conformation with potential relevance to that of membrane-bound IgE in the B cell receptor for antigen. Unlike the earlier, clinically approved anti-IgE antibody omalizumab, 8D6 inhibits binding to FcεRI but not CD23; the structure reveals how this discrimination is achieved through both orthosteric and allosteric mechanisms, supporting therapeutic strategies that retain the benefits of CD23 binding.

Journal Keywords: Immunology

Subject Areas: Biology and Bio-materials, Medicine


Instruments: I04-1-Macromolecular Crystallography (fixed wavelength) , I04-Macromolecular Crystallography

Added On: 06/08/2018 11:56

Documents:
s41598-018-29664-4.pdf

Discipline Tags:

Non-Communicable Diseases Health & Wellbeing Structural biology Drug Discovery Life Sciences & Biotech Allergic Diseases

Technical Tags:

Diffraction Macromolecular Crystallography (MX)