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Ycf48 involved in the biogenesis of the oxygen-evolving photosystem II complex is a seven-bladed beta-propeller protein

DOI: 10.1073/pnas.1800609115 DOI Help

Authors: Jianfeng Yu (Imperial College London) , Jana Knoppová (Institute of Microbiology, Czech Academy of Sciences) , Franck Michoux (Imperial College London) , Wojciech Bialek (Imperial College London) , Ernesto Cota (Imperial College London) , Mahendra K. Shukla (Institute of Microbiology, Czech Academy of Sciences; University of South Bohemia) , Adéla Strašková (Institute of Microbiology, Czech Academy of Sciences) , Guillem Pascual Aznar (Institute of Microbiology, Czech Academy of Sciences; University of South Bohemia) , Roman Sobotka (Institute of Microbiology, Czech Academy of Sciences; University of South Bohemia) , Josef Komenda (Institute of Microbiology, Czech Academy of Sciences; University of South Bohemia) , James W. Murray (Imperial College London) , Peter J. Nixon (Imperial College London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Proceedings Of The National Academy Of Sciences , VOL 49

State: Published (Approved)
Published: July 2018
Diamond Proposal Number(s): 7299 , 12579

Abstract: Robust photosynthesis in chloroplasts and cyanobacteria requires the participation of accessory proteins to facilitate the assembly and maintenance of the photosynthetic apparatus located within the thylakoid membranes. The highly conserved Ycf48 protein acts early in the biogenesis of the oxygen-evolving photosystem II (PSII) complex by binding to newly synthesized precursor D1 subunit and by promoting efficient association with the D2 protein to form a PSII reaction center (PSII RC) assembly intermediate. Ycf48 is also required for efficient replacement of damaged D1 during the repair of PSII. However, the structural features underpinning Ycf48 function remain unclear. Here we show that Ycf48 proteins encoded by the thermophilic cyanobacterium Thermosynechococcus elongatus and the red alga Cyanidioschyzon merolae form seven-bladed beta-propellers with the 19-aa insertion characteristic of eukaryotic Ycf48 located at the junction of blades 3 and 4. Knowledge of these structures has allowed us to identify a conserved “Arg patch” on the surface of Ycf48 that is important for binding of Ycf48 to PSII RCs but also to larger complexes, including trimeric photosystem I (PSI). Reduced accumulation of chlorophyll in the absence of Ycf48 and the association of Ycf48 with PSI provide evidence of a more wide-ranging role for Ycf48 in the biogenesis of the photosynthetic apparatus than previously thought. Copurification of Ycf48 with the cyanobacterial YidC protein insertase supports the involvement of Ycf48 during the cotranslational insertion of chlorophyll-binding apopolypeptides into the membrane.

Journal Keywords: photosystem II; photosynthesis; chlorophyll-binding proteins

Subject Areas: Biology and Bio-materials


Instruments: I03-Macromolecular Crystallography , I04-Macromolecular Crystallography

Other Facilities: ESRF

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