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Structures of the fungal dynamin-related protein Vps1 reveal a unique, open helical architecture

DOI: 10.1083/jcb.201712021 DOI Help

Authors: Natalia V. Varlakhanova (University of Pittsburgh School of Medicine) , Frances J. D. Alvarez (University of Pittsburgh School of Medicine) , Tyler M. Brady (University of Pittsburgh School of Medicine) , Bryan A. Tornabene (University of Pittsburgh School of Medicine) , Christopher J. Hosford (Cornell University) , Joshua S. Chappie (Cornell University) , Peijun Zhang (University of Pittsburgh School of Medicine; Diamond Light Source; Wellcome Trust Centre for Human Genetics, University of Oxford) , Marijn G. J. Ford (University of Pittsburgh)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: The Journal Of Cell Biology , VOL 14

State: Published (Approved)
Published: August 2018
Diamond Proposal Number(s): 14856

Abstract: Dynamin-related proteins (DRPs) are large multidomain GTPases required for diverse membrane-remodeling events. DRPs self-assemble into helical structures, but how these structures are tailored to their cellular targets remains unclear. We demonstrate that the fungal DRP Vps1 primarily localizes to and functions at the endosomal compartment. We present crystal structures of a Vps1 GTPase–bundle signaling element (BSE) fusion in different nucleotide states to capture GTP hydrolysis intermediates and concomitant conformational changes. Using cryoEM, we determined the structure of full-length GMPPCP-bound Vps1. The Vps1 helix is more open and flexible than that of dynamin. This is due to further opening of the BSEs away from the GTPase domains. A novel interface between adjacent GTPase domains forms in Vps1 instead of the contacts between the BSE and adjacent stalks and GTPase domains as seen in dynamin. Disruption of this interface abolishes Vps1 function in vivo. Hence, Vps1 exhibits a unique helical architecture, highlighting structural flexibilities of DRP self-assembly.

Subject Areas: Biology and Bio-materials

Diamond Offline Facilities: Electron Bio-Imaging Centre (eBIC)
Instruments: Krios I-Titan Krios I at Diamond

Added On: 10/08/2018 11:00

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