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Fate of liposomes in the presence of phospholipase C and D: From atomic to supramolecular lipid arrangement

DOI: 10.1021/acscentsci.8b00286 DOI Help

Authors: Margaret N. Holme (Imperial College London; Karolinska Institutet) , M. Harunur Rashid (RMIT University) , Michael R. Thomas (Imperial College London) , Hanna M. G. Barriga (Karolinska Institutet) , Karla−luise Herpoldt (Imperial College London) , Richard K. Heenan (STFC ISIS Facility) , Cecile A. Dreiss (King's College London) , José Leobardo Bañuelos (STFC ISIS Facility; The University of Texas at El Paso) , Hai-nan Xie (Imperial College London) , Irene Yarovsky (RMIT University) , Molly M. Stevens (Imperial College London; Karolinska Institutet)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acs Central Science

State: Published (Approved)
Published: August 2018
Diamond Proposal Number(s): 14792

Open Access Open Access

Abstract: Understanding the origins of lipid membrane bilayer rearrangement in response to external stimuli is an essential component of cell biology and the bottom-up design of liposomes for biomedical applications. The enzymes phospholipase C and D (PLC and PLD) both cleave the phosphorus–oxygen bonds of phosphate esters in phosphatidylcholine (PC) lipids. The atomic position of this hydrolysis reaction has huge implications for the stability of PC-containing self-assembled structures, such as the cell wall and lipid-based vesicle drug delivery vectors. While PLC converts PC to diacylglycerol (DAG), the interaction of PC with PLD produces phosphatidic acid (PA). Here we present a combination of small-angle scattering data and all-atom molecular dynamics simulations, providing insights into the effects of atomic-scale reorganization on the supramolecular assembly of PC membrane bilayers upon enzyme-mediated incorporation of DAG or PA. We observed that PC liposomes completely disintegrate in the presence of PLC, as conversion of PC to DAG progresses. At lower concentrations, DAG molecules within fluid PC bilayers form hydrogen bonds with backbone carbonyl oxygens in neighboring PC molecules and burrow into the hydrophobic region. This leads initially to membrane thinning followed by a swelling of the lamellar phase with increased DAG. At higher DAG concentrations, localized membrane tension causes a change in lipid phase from lamellar to the hexagonal and micellar cubic phases. Molecular dynamics simulations show that this destabilization is also caused in part by the decreased ability of DAG-containing PC membranes to coordinate sodium ions. Conversely, PLD-treated PC liposomes remain stable up to extremely high conversions to PA. Here, the negatively charged PA headgroup attracts significant amounts of sodium ions from the bulk solution to the membrane surface, leading to a swelling of the coordinated water layer. These findings are a vital step toward a fundamental understanding of the degradation behavior of PC lipid membranes in the presence of these clinically relevant enzymes, and toward the rational design of diagnostic and drug delivery technologies for phospholipase-dysregulation-based diseases.

Subject Areas: Biology and Bio-materials, Chemistry, Medicine

Instruments: I22-Small angle scattering & Diffraction

Other Facilities: ISIS