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Correlation of cryo soft x-ray tomography with cryo fluorescence microscopy to characterise cellular organelles at beamline B24, Diamond Light Source

DOI: 10.1017/S1431927618014162 DOI Help

Authors: Matthew C. Spink (Diamond Light Source) , Michele C. Darrow (Diamond Light Source) , Hannah Fisher (Diamond Light Source) , Alister Burt (Diamond Light Source) , Karen Marshall (University of Sussex) , Imanol Luengo (Diamond Light Source) , Maria Harkiolaki (Diamond Light Source) , Liz Duke (Diamond Light Source)
Co-authored by industrial partner: No

Type: Conference Paper
Conference: Microscopy & Microanalysis 2018
Peer Reviewed: No

State: Published (Approved)
Published: August 2018

Abstract: Cryo soft-X-ray tomography (Cryo–SXT) is a 3D imaging technique that allows us to image whole vitrified biological cells and their contents in a near native state. Cryo-SXT utilises the natural contrast of cellular structures by imaging in an energy range termed the ‘water window’ The water window is between the K absorption edges of Carbon 284 eV and Oxygen 543 eV. Imaging between these energies results in higher X-ray absorption by carbon rich cellular structures than oxygen containing molecules such as vitrified water. The resulting contrast allows us to see cellular structures (Figure 1A) at a resolution of ~40nm, depending on the sample and microscope components.

Subject Areas: Biology and Bio-materials


Instruments: B24-Cryo Soft X-ray Tomography