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Structure-function correlative microscopy of peritubular and intertubular dentine

DOI: 10.3390/ma11091493 DOI Help

Authors: Tan Sui (University of Surrey) , Jiří Dluhoš (TESCAN Brno) , Tao Li (National University of Singapore) , Kaiyang Zeng (National University of Singapore) , Adrian Cernescu (Neaspec GmbH) , Gabriel Landini (University of Birmingham) , Alexander Korsunsky (University of Oxford)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Materials , VOL 11

State: Published (Approved)
Published: August 2018
Diamond Proposal Number(s): 11831 , 21312

Open Access Open Access

Abstract: Peritubular dentine (PTD) and intertubular dentine (ITD) were investigated by 3D correlative Focused Ion Beam (FIB)-Scanning Electron Microscopy (SEM)-Energy Dispersive Spectroscopy (EDS) tomography, tapping mode Atomic Force Microscopy (AFM) and scattering-type Scanning Near-Field Optical Microscopy (s-SNOM) mapping. The brighter appearance of PTD in 3D SEM-Backscattered-Electron (BSE) imaging mode and the corresponding higher grey value indicate a greater mineral concentration in PTD (~160) compared to ITD (~152). However, the 3D FIB-SEM-EDS reconstruction and high resolution, quantitative 2D map of the Ca/P ratio (~1.8) fail to distinguish between PTD and ITD. This has been further confirmed using nanoscale 2D AFM map, which clearly visualised biopolymers and hydroxyapatite (HAp) crystallites with larger mean crystallite size in ITD (32 ± 8 nm) than that in PTD (22 ± 3 nm). Correlative microscopy reveals that the principal difference between PTD and ITD arises primarily from the nanoscale packing density of the crystallites bonded together by thin biopolymer, with moderate contribution from the chemical composition difference. The structural difference results in the mechanical properties variation that is described by the parabolic stiffness-volume fraction correlation function introduced here. The obtained results benefit a microstructure-based mechano-chemical model to simulate the chemical etching process that can occur in human dental caries and some of its treatments.

Journal Keywords: peritubular dentine (PTD); intertubular dentine (ITD); FIB-SEM-EDS tomography; tapping mode AFM; s-SNOM; parabolic stiffness-volume fraction correlation function

Subject Areas: Biology and Bio-materials


Instruments: B16-Test Beamline

Added On: 06/09/2018 09:07

Documents:
materials-11-01493.pdf

Discipline Tags:

Dentistry Health & Wellbeing Life Sciences & Biotech

Technical Tags:

Diffraction