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Megahertz serial crystallography

DOI: 10.1038/s41467-018-06156-7 DOI Help

Authors: Max O. Wiedorn (Deutsches Elektronen-Synchrotron DESY; Universität Hamburg) , Dominik Oberthuer (Deutsches Elektronen-Synchrotron DESY) , Richard Bean (European XFEL) , Robin Schubert (Universität Hamburg; European XFEL) , Nadine Werner (Universität Hamburg) , Brian Abbey (La Trobe University) , Martin Aepfelbacher (University Medical Center Hamburg-Eppendorf (UKE)) , Luigi Adriano (Deutsches Elektronen-Synchrotron DESY) , Aschkan Allahgholi (Deutsches Elektronen-Synchrotron DESY) , Nasser Al-qudami (European XFEL) , Jakob Andreasson (Uppsala University; Institute of Physics of the Czech Academy of Sciences; Chalmers University of Technology) , Steve Aplin (Deutsches Elektronen-Synchrotron DESY) , Salah Awel (Deutsches Elektronen-Synchrotron DESY; Universität Hamburg) , Kartik Ayyer (Deutsches Elektronen-Synchrotron DESY) , Saša Bajt (Deutsches Elektronen-Synchrotron DESY) , Imrich Barák (Institute of Molecular Biology, SAS) , Sadia Bari (Deutsches Elektronen-Synchrotron DESY) , Johan Bielecki (European XFEL) , Sabine Botha (Universität Hamburg) , Djelloul Boukhelef (European XFEL) , Wolfgang Brehm (Deutsches Elektronen-Synchrotron DESY) , Sandor Brockhauser (European XFEL; Hungarian Academy of Sciences) , Igor Cheviakov (University Medical Center Hamburg-Eppendorf (UKE)) , Matthew A. Coleman (Lawrence Livermore National Laboratory) , Francisco Cruz-mazo (Universidad de Sevilla) , Cyril Danilevski (European XFEL) , Connie Darmanin (La Trobe University) , R. Bruce Doak (Max Planck Institute for Medical Research) , Martin Domaracky (Deutsches Elektronen-Synchrotron DESY) , Katerina Dörner (European XFEL) , Yang Du (Deutsches Elektronen-Synchrotron DESY) , Hans Fangohr (European XFEL; University of Southampton) , Holger Fleckenstein (Deutsches Elektronen-Synchrotron DESY) , Matthias Frank (Lawrence Livermore National Laboratory) , Petra Fromme (Arizona State University) , Alfonso M. Gañán-calvo (Universidad de Sevilla) , Yaroslav Gevorkov (Deutsches Elektronen-Synchrotron DESY; Hamburg University of Technology) , Klaus Giewekemeyer (European XFEL) , Helen Mary Ginn (University of Oxford; Diamond Light Source) , Heinz Graafsma (Deutsches Elektronen-Synchrotron DESY; Mid Sweden University) , Rita Graceffa (European XFEL) , Dominic Greiffenberg (Paul Scherrer Institut) , Lars Gumprecht (Deutsches Elektronen-Synchrotron DESY) , Peter Göttlicher (Deutsches Elektronen-Synchrotron DESY) , Janos Hajdu (Uppsala University; Institute of Physics of the Czech Academy of Sciences) , Steffen Hauf (European XFEL) , Michael Heymann (Max Planck Institute of Biochemistry) , Susannah Holmes (La Trobe University) , Daniel A. Horke (Deutsches Elektronen-Synchrotron DESY; Universität Hamburg) , Mark S. Hunter (Linac Coherent Light Source, SLAC National Accelerator Laboratory) , Siegfried Imlau (Deutsches Elektronen-Synchrotron DESY) , Alexander Kaukher (European XFEL) , Yoonhee Kim (European XFEL) , Alexander Klyuev (Deutsches Elektronen-Synchrotron DESY) , Juraj Knoška (Deutsches Elektronen-Synchrotron DESY; Universität Hamburg) , Bostjan Kobe (University of Queensland) , Manuela Kuhn (Deutsches Elektronen-Synchrotron DESY) , Christopher Kupitz (University of Wisconsin-Milwaukee) , Jochen Küpper (Deutsches Elektronen-Synchrotron DESY; Universität Hamburg) , Janine Mia Lahey-rudolph (Deutsches Elektronen-Synchrotron DESY; University of Lübeck) , Torsten Laurus (Deutsches Elektronen-Synchrotron DESY) , Karoline Le Cong (Universität Hamburg) , Romain Letrun (European XFEL) , P. Lourdu Xavier (Deutsches Elektronen-Synchrotron DESY; Max-Planck Institute for the Structure and Dynamics of Matter) , Luis Maia (European XFEL) , Filipe R. N. C. Maia (Uppsala University; NERSC, Lawrence Berkeley National Laboratory) , Valerio Mariani (Deutsches Elektronen-Synchrotron DESY) , Marc Messerschmidt (European XFEL) , Markus Metz (Deutsches Elektronen-Synchrotron DESY) , Davide Mezza (Paul Scherrer Institut) , Thomas Michelat (European XFEL) , Grant Mills (European XFEL) , Diana C. F. Monteiro (Universität Hamburg) , Andrew Morgan (Deutsches Elektronen-Synchrotron DESY) , Kerstin Mühlig (Uppsala University) , Anna Munke (Uppsala University) , Astrid Münnich (European XFEL) , Julia Nette (Universität Hamburg) , Keith A. Nugent (La Trobe University) , Theresa Nuguid (Universität Hamburg) , Allen M. Orville (Diamond Light Source) , Suraj Pandey (University of Wisconsin-Milwaukee) , Gisel Pena (Deutsches Elektronen-Synchrotron DESY) , Pablo Villanueva-perez (Deutsches Elektronen-Synchrotron DESY) , Jennifer Poehlsen (Deutsches Elektronen-Synchrotron DESY) , Gianpietro Previtali (European XFEL) , Lars Redecke (University Medical Center Hamburg-Eppendorf (UKE); University of Lübeck) , Winnie Maria Riekehr (University of Lübeck) , Holger Rohde (University Medical Center Hamburg-Eppendorf (UKE)) , Adam Round (European XFEL) , Tatiana Safenreiter (Deutsches Elektronen-Synchrotron DESY) , Iosifina Sarrou (Deutsches Elektronen-Synchrotron DESY) , Tokushi Sato (Deutsches Elektronen-Synchrotron DESY) , Marius Schmidt (University of Wisconsin-Milwaukee) , Bernd Schmitt (Paul Scherrer Institut) , Robert Schönherr (University of Lübeck) , Joachim Schulz (European XFEL) , Jonas A. Sellberg (KTH Royal Institute of Technology) , M. Marvin Seibert (Uppsala University) , Carolin Seuring (Deutsches Elektronen-Synchrotron DESY; Universität Hamburg) , Megan L. Shelby (Lawrence Livermore National Laboratory) , Robert L. Shoeman (Max Planck Institute for Medical Research) , Marcin Sikorski (European XFEL) , Alessandro Silenzi (European XFEL) , Claudiu A. Stan (Rutgers University Newark) , Xintian Shi (Paul Scherrer Institut) , Stephan Stern (Deutsches Elektronen-Synchrotron DESY; European XFEL) , Jola Sztuk-dambietz (European XFEL) , Janusz Szuba (European XFEL) , Aleksandra Tolstikova (Deutsches Elektronen-Synchrotron DESY) , Martin Trebbin (Universität Hamburg; University at Buffalo) , Ulrich Trunk (Deutsches Elektronen-Synchrotron DESY) , Patrik Vagovic (Deutsches Elektronen-Synchrotron DESY; European XFEL) , Thomas Ve (Griffith University) , Britta Weinhausen (European XFEL) , Thomas A. White (Deutsches Elektronen-Synchrotron DESY) , Krzysztof Wrona (European XFEL) , Chen Xu (European XFEL) , Oleksandr Yefanov (Deutsches Elektronen-Synchrotron DESY) , Nadia Zatsepin (Arizona State University) , Jiaguo Zhang (Paul Scherrer Institut) , Markus Perbandt (Universität Hamburg; University Medical Center Hamburg-Eppendorf (UKE)) , Adrian P. Mancuso (European XFEL) , Christian Betzel (Universität Hamburg; Integrated Biology Infrastructure Life-Science Facility at the European XFEL (XBI)) , Henry Chapman (Deutsches Elektronen-Synchrotron DESY; Universität Hamburg) , Anton Barty (Deutsches Elektronen-Synchrotron DESY)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Communications , VOL 9

State: Published (Approved)
Published: October 2018

Open Access Open Access

Abstract: The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.

Journal Keywords: Imaging techniques; Nanocrystallography

Subject Areas: Chemistry

Facility: European XFEL

Documents:
s41467-018-06156-7.pdf